Zhang Yuan-Yuan, Li Hong-Kun, Huang Xiao, Yuan Yu-Jiao, Zhang Xiao-Fei, Gao Xin-Sheng, Wang Xiang-Jun, Wei Ming-Ming, Huang Hua-Sun, Li Weiguo
State Key Laboratory of Tropical Crop Breeding, State Centre for Rubber Breeding, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China.
Dehong Institute of Tropical Agricultural Sciences of Yunnan Province, Ruili, 678600, Yunnan, China.
Plant Reprod. 2024 Mar;37(1):47-56. doi: 10.1007/s00497-023-00481-8. Epub 2023 Sep 27.
Unreduced megagametophytes via second-division restitution were confirmed through heterozygosity analysis, and four candidate physical centromeres of rubber were located for the first time. The evaluation of maternal heterozygosity restitution (MHR) is vital in identifying the mechanism of 2n gametogenesis and assessing the utilization value of 2n gametes. In this study, three full-sib triploid populations were employed to evaluate the MHR of 2n female gametes of rubber tree clone GT1 and to confirm their genetic derivation. The 2n female gametes of GT1 were derived from second-division restitution (SDR) and transmitted more than half of the parental heterozygosity. In addition, low recombination frequency markers were developed, and four candidate physical centromeres of rubber tree were located for the first time. The confirmation that 2n female gametes of rubber tree clone GT1 are derived from SDR provides insights into the molecular mechanisms of 2n gametogenesis. In addition, the identified centromere location will aid in the development of centromeric markers for the rapid identification of the 2n gametogenesis mechanism.
通过杂合性分析证实了经第二次分裂 restitution 产生的未减数雌配子体，并首次定位了橡胶树的四个候选物理着丝粒。母本杂合性 restitution（MHR）的评估对于确定 2n 配子发生机制和评估 2n 配子的利用价值至关重要。在本研究中，利用三个全同胞三倍体群体评估橡胶树克隆 GT1 的 2n 雌配子的 MHR，并确认其遗传来源。GT1 的 2n 雌配子源自第二次分裂 restitution（SDR），并传递了超过一半的亲本杂合性。此外，开发了低重组频率标记，并首次定位了橡胶树的四个候选物理着丝粒。橡胶树克隆 GT1 的 2n 雌配子源自 SDR 的确认，为 2n 配子发生的分子机制提供了见解。此外，确定的着丝粒位置将有助于开发着丝粒标记，以快速鉴定 2n 配子发生机制。
