Institut National de Recherche pour l'Agriculture et l'Environnement (INRAE), Micalis Institut, AgroParisTech, University of Paris-Saclay, 78350 Jouy-en-Josas, France.
INDIGO Therapeutics, 33000 Bordeaux, France.
Biomolecules. 2023 Aug 24;13(9):1295. doi: 10.3390/biom13091295.
Intestinal barrier integrity is essential in order to maintain the homeostasis of mucosal functions and efficient defensive reactions against chemical and microbial challenges. An impairment of the intestinal barrier has been observed in several chronic diseases. The gut microbiota and its impact on intestinal homeostasis is well described and numerous studies suggest the ability of some probiotic strains to protect the intestinal epithelial integrity and host homeostasis. In this work, we aimed to assess the beneficial effects of three strains ( LR04, LC03, and CNCM I-4459) and their mechanism of action in low-grade inflammation or neonatal maternal separation models in mice. We compared the impact of these strains to that of the well-known probiotic GG. Our results demonstrated that the three strains have the potential to restore the barrier functions by (i) increasing mucus production, (ii) restoring normal permeability, and (iii) modulating colonic hypersensitivity. Moreover, gene expression analysis of junctional proteins revealed the implication of Claudin 2 and Cingulin in the mechanisms that underlie the interactions between the strains and the host. Taken together, our data suggest that LR04, CNCM I-4459, and LC03 restore the functions of an impaired intestinal barrier.
肠道屏障的完整性对于维持黏膜功能的体内平衡和有效抵御化学和微生物挑战至关重要。在几种慢性疾病中都观察到肠道屏障受损。肠道微生物群及其对肠道内稳态的影响已有详细描述，许多研究表明，一些益生菌菌株能够保护肠道上皮细胞的完整性和宿主的内稳态。在这项工作中，我们旨在评估三种菌株（LR04、LC03 和 CNCM I-4459）及其在低水平炎症或新生期母体分离模型小鼠中的作用机制的有益效果，并将其与著名的益生菌 GG 进行比较。我们的结果表明，这三种菌株具有恢复屏障功能的潜力，其机制包括：（i）增加黏液的产生；（ii）恢复正常通透性；（iii）调节结肠高敏性。此外，对连接蛋白的基因表达分析表明，Claudin 2 和 Cingulin 参与了菌株与宿主相互作用的机制。综上所述，我们的数据表明，LR04、CNCM I-4459 和 LC03 恢复了受损肠道屏障的功能。
