College of Life Sciences, Sichuan University, Chengdu 610044, China.
Key Laboratory of Bio-Resource and Eco-Environment of the Ministry of Education, Chengdu 610064, China.
Biomolecules. 2023 Sep 6;13(9):1353. doi: 10.3390/biom13091353.
is a foodborne pathogen that poses a serious threat to both human and animal health and food safety. Flaxseed is rich in unsaturated fatty acids; has anti-metabolic syndrome, anti-inflammatory, and neuroprotective properties; and may be a potential source of feed additives. To investigate the impact of flaxseed on -infected laying hens, we administered subsp. serovar Enteritidis ( Enteritidis) after adding flaxseed to the feed of laying hens (15% [750 mg/kg]). Enteritidis colonization was reduced and its clearance was accelerated from the laying hens. Furthermore, flaxseed supplementation mitigated the damage to the ileum caused by Enteritidis. We analyzed alterations in intestinal flora through 16S rRNA amplicon sequencing. Enteritidis infection increased the abundance of and triggered the host inflammatory response. Conversely, the addition of flaxseed to the feed increased the abundance of beneficial intestinal bacteria, such as and . Ovarian health is important for egg production performance in laying hens and our findings indicate that Enteritidis can persist in the ovaries for an extended period. Therefore, we further performed transcriptome sequencing analysis of ovarian tissues on day seven after Enteritidis infection. Enteritidis infection leads to altered ovarian gene expression, including the downregulation of lipid metabolism and growth and development genes and the upregulation of host immune response genes in laying hens. The upregulation of genes associated with growth and development may have stimulated ovarian growth and development.
是一种食源性致病菌，对人类和动物健康以及食品安全构成严重威胁。亚麻籽富含不饱和脂肪酸；具有抗代谢综合征、抗炎和神经保护作用；并且可能是饲料添加剂的潜在来源。为了研究亚麻籽对感染的蛋鸡的影响，我们在蛋鸡饲料中添加亚麻籽（15%[750mg/kg]）后，用 subsp. serovar Enteritidis（肠炎沙门氏菌）进行攻毒。肠炎沙门氏菌的定植减少，从蛋鸡中清除加速。此外，亚麻籽补充剂减轻了肠炎沙门氏菌对回肠造成的损害。我们通过 16S rRNA 扩增子测序分析了肠道菌群的变化。肠炎沙门氏菌感染增加了 和 的丰度，并引发了宿主炎症反应。相反，在饲料中添加亚麻籽增加了有益肠道细菌的丰度，如 和 。卵巢健康对蛋鸡的产蛋性能很重要，我们的研究结果表明，肠炎沙门氏菌可以在卵巢中持续存在很长时间。因此，我们在感染肠炎沙门氏菌后第七天进一步对卵巢组织进行了转录组测序分析。肠炎沙门氏菌感染导致卵巢基因表达发生改变，包括脂质代谢和生长发育基因下调以及宿主免疫反应基因上调。与生长发育相关的基因上调可能刺激了卵巢的生长和发育。
