Regulates the Function of Lympho-Myeloid Primed Progenitors after Transplantation.

, a zinc finger E-box binding homeobox epithelial-mesenchymal (EMT) transcription factor, acts as a critical regulator of hematopoietic stem cell (HSC) self-renewal and multi-lineage differentiation. Whether directly regulates the function of multi-potent progenitors primed for hematopoietic lineage commitment remains ill defined. By using an inducible conditional mouse model where was genetically engineered to be deficient in the adult hematopoietic system (hereafter ), we found that the absolute cell number of immunophenotypically defined lympho-myeloid primed progenitors (LMPPs) from mice was reduced. Myeloid- and lymphoid-biased HSCs in mice were unchanged, implying that defective LMPP generation from mice was not directly caused by an imbalance of lineage-biased HSCs. Functional analysis of LMPP from mice, as judged by competitive transplantation, revealed an overall reduction in engraftment to hematopoietic organs over 4 weeks, which correlated with minimal T-cell engraftment, reduced B-cell and monocyte/macrophage engraftment, and unperturbed granulocyte engraftment. Thus, regulates LMPP differentiation potential to select lympho-myeloid lineages in the context of transplantation.