Hyun Jimin, Kang Sang-In, Lee Sang-Woon, Amarasiri R P G S K, Nagahawatta D P, Roh Yujin, Wang Lei, Ryu Bomi, Jeon You-Jin
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.
Seafood Research Center, Silla University, Busan 49277, Republic of Korea.
Antioxidants (Basel). 2023 Sep 13;12(9):1755. doi: 10.3390/antiox12091755.
Olive flounder (OF) is a widely aqua-cultivated and recognized socioeconomic resource in Korea. However, more than 50% of by-products are generated when processing one OF, and there is no proper way to utilize them. With rising awareness and interest in eco-friendly bio-materialization recycling, this research investigates the potential of enzymatic hydrolysis of OF by-products (OFB) to produce functional ingredients. Various enzymatic hydrolysates of OFB (OFBEs) were generated using 11 commercial enzymes. Among them, Prozyme 2000P-assisted OFBE (OFBP) exhibited the highest protein content and yield, as well as low molecularization. The muscle regenerative potential of OFBEs was evaluated using C2C12 myoblasts, revealing that OFBP positively regulated myoblast differentiation. In an in vitro Dex-induced myotube atrophy model, OFBP protected against muscle atrophy and restored myotube differentiation and Dex-induced reactive oxygen species (ROS) production. Furthermore, zebrafish treated with OFBEs showed improved locomotor activity and body weight, with OFBP exhibiting outstanding restoration in the Dex-induced muscle atrophy zebrafish in vivo model. In conclusion, OFBEs, particularly OFBP, produce hydrolysates with enhanced physiological usability and muscle regenerative potential. Further research on its industrial application and mechanistic insights is needed to realize its potential as a high-quality protein food ingredient derived from OF processing by-products.
牙鲆是韩国广泛养殖且具有重要社会经济价值的资源。然而，加工一条牙鲆时会产生超过50%的副产品，且尚无妥善利用这些副产品的方法。随着人们对生态友好型生物材料回收利用的意识和兴趣不断提高，本研究探讨了牙鲆副产品（OFB）酶解生产功能成分的潜力。使用11种商业酶制备了各种牙鲆副产品酶解物（OFBEs）。其中，Prozyme 2000P辅助的牙鲆副产品酶解物（OFBP）蛋白质含量和产率最高，且分子质量较小。利用C2C12成肌细胞评估了OFBEs的肌肉再生潜力，结果显示OFBP对成肌细胞分化具有正向调节作用。在体外地塞米松（Dex）诱导的肌管萎缩模型中，OFBP可预防肌肉萎缩，恢复肌管分化，并减少Dex诱导的活性氧（ROS）生成。此外，用OFBEs处理的斑马鱼运动活性和体重有所改善，在Dex诱导的体内肌肉萎缩斑马鱼模型中，OFBP表现出显著的恢复效果。总之，OFBEs，尤其是OFBP，可产生具有更高生理可用性和肌肉再生潜力的水解产物。需要进一步研究其工业应用和作用机制，以实现其作为牙鲆加工副产品衍生的优质蛋白质食品成分的潜力。
