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使用高密度自体软骨细胞植入术（HD-ACI）治疗软骨缺损

Cartilage Defect Treatment Using High-Density Autologous Chondrocyte Implantation (HD-ACI).

作者信息

Guillén-García Pedro, Guillén-Vicente Isabel, Rodríguez-Iñigo Elena, Guillén-Vicente Marta, Fernández-Jaén Tomás Fernando, Navarro Ramón, Aboli Lucía, Torres Raúl, Abelow Steve, López-Alcorocho Juan Manuel

机构信息

Department of Traumatology and Research Unit, Clínica CEMTRO, 28035 Madrid, Spain.

出版信息

Bioengineering (Basel). 2023 Sep 13;10(9):1083. doi: 10.3390/bioengineering10091083.

DOI:10.3390/bioengineering10091083
PMID:37760185
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10525711/
Abstract

Hyaline cartilage's inability to self-repair can lead to osteoarthritis and joint replacement. Various treatments, including cell therapy, have been developed for cartilage damage. Autologous chondrocyte implantation (ACI) is considered the best option for focal chondral lesions. In this article, we aimed to create a narrative review that highlights the evolution and enhancement of our chondrocyte implantation technique: High-Density-ACI (HD-ACI) Membrane-assisted Autologous Chondrocyte Implantation (MACI) improved ACI using a collagen membrane as a carrier. However, low cell density in MACI resulted in softer regenerated tissue. HD-ACI was developed to improve MACI, implanting 5 million chondrocytes per cm, providing higher cell density. In animal models, HD-ACI formed hyaline-like cartilage, while other treatments led to fibrocartilage. HD-ACI was further evaluated in patients with knee or ankle defects and expanded to treat hip lesions and bilateral defects. HD-ACI offers a potential solution for cartilage defects, improving outcomes in regenerative medicine and cell therapy. HD-ACI, with its higher cell density, shows promise for treating chondral defects and advancing cartilage repair in regenerative medicine and cell therapy.

摘要

透明软骨无法自我修复会导致骨关节炎和关节置换。针对软骨损伤已开发出多种治疗方法，包括细胞疗法。自体软骨细胞植入术（ACI）被认为是治疗局灶性软骨损伤的最佳选择。在本文中，我们旨在进行一项叙述性综述，重点介绍我们的软骨细胞植入技术的演变与改进：高密度自体软骨细胞植入术（HD-ACI）。膜辅助自体软骨细胞植入术（MACI）使用胶原膜作为载体改进了ACI。然而，MACI中的细胞密度较低，导致再生组织较软。HD-ACI的开发是为了改进MACI，每平方厘米植入500万个软骨细胞，提供更高的细胞密度。在动物模型中，HD-ACI形成了透明样软骨，而其他治疗方法则导致纤维软骨形成。HD-ACI在膝关节或踝关节缺损患者中得到进一步评估，并扩展至治疗髋关节损伤和双侧缺损。HD-ACI为软骨缺损提供了一种潜在的解决方案，改善了再生医学和细胞治疗的效果。HD-ACI具有更高的细胞密度，有望治疗软骨缺损，并在再生医学和细胞治疗中推动软骨修复。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/3f38952b5f6f/bioengineering-10-01083-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/357a37a0c201/bioengineering-10-01083-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/760846f717d3/bioengineering-10-01083-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/3f38952b5f6f/bioengineering-10-01083-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/357a37a0c201/bioengineering-10-01083-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/37c5bfbb44cb/bioengineering-10-01083-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/dadb47ae2429/bioengineering-10-01083-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/330e9af3a507/bioengineering-10-01083-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/848d4ac0311a/bioengineering-10-01083-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/12389cd5e7ef/bioengineering-10-01083-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dd9/10525711/3f38952b5f6f/bioengineering-10-01083-g008.jpg

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