Saruuljavkhlan Batsaikhan, Alfaray Ricky Indra, Oyuntsetseg Khasag, Gantuya Boldbaatar, Khangai Ayush, Renchinsengee Namsrai, Matsumoto Takashi, Akada Junko, Azzaya Dashdorj, Davaadorj Duger, Yamaoka Yoshio
Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu 879-5593, Oita, Japan.
Helicobacter pylori and Microbiota Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60286, East Java, Indonesia.
Cancers (Basel). 2023 Sep 12;15(18):4528. doi: 10.3390/cancers15184528.
The prevalence of gastric cancer in Mongolia, in East Asia, remains the highest in the world. However, most strains in Mongolia have a less virulent Western-type CagA. We aimed to determine how genomic variation affected gastric diseases, especially gastric cancer, based on comprehensive genome analysis.
We identified a set of 274 virulence-associated genes in , including virulence factor and outer membrane protein (OMP) genes, the type four secretion system gene cluster, and 13 well-known virulence gene genotypes in 223 strains and their associations with gastric cancer and other gastric diseases. We conducted a genome-wide association study on 158 strains (15 gastric cancer and 143 non-gastric cancer strains).
Out of 274 genes, we found 13 genes were variable depending on disease outcome, especially iron regulating OMP genes. strains from Mongolia were divided into two main subgroups: subgroup (Sg1) with high risk and Sg2 with low risk for gastric cancer. The general characteristics of Sg1 strains are that they possess more virulence genotype genes. We found nine non-synonymous single nucleotide polymorphisms in seven genes that are linked with gastric cancer strains.
Highly virulent strains may adapt through host-influenced genomic variations, potentially impacting gastric carcinogenesis.
在东亚的蒙古国,胃癌的患病率仍然是世界上最高的。然而,蒙古国的大多数菌株具有毒性较低的西方型细胞毒素相关基因A(CagA)。我们旨在基于全面的基因组分析,确定基因组变异如何影响胃部疾病,尤其是胃癌。
我们在223株幽门螺杆菌中鉴定出一组274个与毒力相关的基因,包括毒力因子和外膜蛋白(OMP)基因、四型分泌系统基因簇,以及13种著名的毒力基因基因型,并研究它们与胃癌和其他胃部疾病的关联。我们对158株幽门螺杆菌(其中15株为胃癌菌株,143株为非胃癌菌株)进行了全基因组关联研究。
在274个基因中,我们发现13个基因因疾病结果而异,尤其是铁调节OMP基因。来自蒙古国的幽门螺杆菌菌株分为两个主要亚组:胃癌高风险亚组(Sg1)和低风险亚组(Sg2)。Sg1菌株的一般特征是它们拥有更多的毒力基因型基因。我们在与胃癌菌株相关的7个基因中发现了9个非同义单核苷酸多态性。
高毒力的幽门螺杆菌菌株可能通过宿主影响的基因组变异进行适应,这可能会影响胃癌的发生。