Han Hsu H
Vet Parasitol. 1986 Oct;21(4):247-54. doi: 10.1016/0304-4017(86)90050-6.
Metacercariae of Fasciola hepatica were labeled with 75Se by exposure of infected Lymnaea columella to 75Se-M, 20 muCi/snail, in 5 ml of water. Seventy-two mice were inoculated with 50 75Se-labeled metacercariae. At intervals after infection, the distribution of metacercariae in gut, body cavities and liver was studied by compressed organ autoradiography, and a variant of this technique adapted to gut content and body cavity washes. Ag degree focus counts from autoradiograms expressed as percentage of actual exposure level were subjected to probit analysis. Estimates of the average departure time of radio-labeled maritas from gastrointestinal tracts and average arrival time into the abdominal cavities were 12.2 and 12.5 h, respectively. The peak accumulation of maritas in abdominal cavities occurred at 24 h post-inoculation. A 7.7-h discrepancy between estimated average departure time from abdominal cavities (92 h) and average arrival time at livers (99.7 h) was attributed to experimental error.
通过将感染的小椎实螺暴露于5毫升水中含20微居里/只蜗牛的75Se-M，对肝片吸虫的后尾蚴进行75Se标记。72只小鼠接种了50个75Se标记的后尾蚴。感染后的不同时间间隔，通过压缩器官放射自显影术研究后尾蚴在肠道、体腔和肝脏中的分布，以及适用于肠道内容物和体腔冲洗液的该技术变体。将放射自显影片上的银颗粒计数表示为实际暴露水平的百分比，并进行概率分析。放射性标记的虫体从胃肠道的平均离开时间和进入腹腔的平均到达时间估计分别为12.2小时和12.5小时。接种后24小时，虫体在腹腔中的积累达到峰值。腹腔估计平均离开时间（92小时）和肝脏平均到达时间（99.7小时）之间7.7小时的差异归因于实验误差。
