Adesiyun A A, Agbonlahor D E, Lombin L H, Kwaga J K
Vet Microbiol. 1986 Sep;12(3):289-94. doi: 10.1016/0378-1135(86)90058-1.
Fourteen strains of Yersinia species isolated from apparently healthy pigs and cattle in Nigeria were screened for four virulence markers using six test systems. These were two in vitro assays, namely, calcium dependency and autoagglutination, both at 37 degrees C, the Serény test in guinea-pigs and the detection of heat-stable enterotoxin (ST) by the rabbit ileal loop test, the ligated intestine test in pigs and the infant mouse system. Seven of the 14 strains of Yersinia were positive for one or more of these tests. Six of nine strains of Y. enterocolitica and one of four Y. intermedia were positive in one or more tests. The only strain of Y. frederiksenii isolated was negative in all six test systems. All three strains of Y. enterocolitica, serotype 0:8 and the only serotype 0:3 isolated were positive in one or more tests. However, only two of five strains of Y. enterocolitica serotype 0:12, 26, the most frequently encountered, were positive. A good correlation was observed between test results of calcium dependency, autoagglutination and Serény assays. The results indicate that cattle and pigs have the potential to transmit virulent strains of Y. enterocolitica to human beings in Nigeria.
使用六种检测系统，对从尼日利亚看似健康的猪和牛中分离出的14株耶尔森氏菌进行了四种毒力标记物的筛选。这些检测系统包括两种体外试验，即37℃下的钙依赖性试验和自凝试验，豚鼠的塞雷尼试验，兔回肠袢试验检测热稳定肠毒素（ST），猪的结扎肠试验和幼鼠系统。14株耶尔森氏菌中有7株对这些检测中的一项或多项呈阳性。9株小肠结肠炎耶尔森氏菌中的6株和4株中间型耶尔森氏菌中的1株在一项或多项检测中呈阳性。分离出的唯一一株弗氏耶尔森氏菌在所有六种检测系统中均为阴性。所有三株0:8血清型小肠结肠炎耶尔森氏菌和分离出的唯一一株0:3血清型菌株在一项或多项检测中呈阳性。然而，最常见的5株0:12、26血清型小肠结肠炎耶尔森氏菌中只有2株呈阳性。钙依赖性试验、自凝试验和塞雷尼试验的检测结果之间观察到良好的相关性。结果表明，在尼日利亚，牛和猪有可能将小肠结肠炎耶尔森氏菌的有毒菌株传播给人类。
