Corominas M, Mestre M, Bas J, Buendia E
Immunology Service, Ciutat Sanitària i Universitària de Bellvitge, L'Hospitalet de Llobregat, Barcelona, Spain.
Clin Exp Immunol. 1998 May;112(2):276-80. doi: 10.1046/j.1365-2249.1998.00570.x.
The low-affinity IgE receptor (FcepsilonRII/CD23) plays a role in IgE production. Cytokines participating in IgE synthesis also modulate CD23 expression on lymphocytes, but whether this modulation is different in atopic subjects remains unclear. We studied CD23 expression on B and T lymphocytes in 10 asthmatic patients with Dermatophagoides pteronyssinus hypersensitivity and 10 healthy non-atopic subjects. Studies were performed by flow cytometry, in phytohaemagglutinin (PHA) or IL-4-stimulated mononuclear cell cultures, alone or in the presence of IFN-gamma. Soluble CD23 (sCD23) released in the culture supernatants was measured by enzyme-linked immunoassay. Both PHA and IL-4 induced the expression of CD23 on lymphocytes of atopic and non-atopic subjects. Whereas PHA increased both the percentage and mean fluorescence intensity of CD23+ B and T cells, IL-4 alone did not increase the percentage of CD23+ T cells. The effects of IFN-gamma were different in both groups, since it was able to reduce the percentage of PHA-stimulated CD23+ T cells only in non-atopic individuals. In non-atopic subjects more than atopic, levels of sCD23 were increased in the supernatants of PHA and IL-4 cultures. These results show that the modulation of CD23 expression is different on B and T cells, and that IFN-gamma acts differently in atopic and non-atopic individuals.
低亲和力IgE受体（FcepsilonRII/CD23）在IgE产生过程中发挥作用。参与IgE合成的细胞因子也可调节淋巴细胞上CD23的表达，但这种调节在特应性个体中是否存在差异尚不清楚。我们研究了10名对尘螨过敏的哮喘患者和10名健康非特应性个体的B淋巴细胞和T淋巴细胞上CD23的表达情况。通过流式细胞术，在单独或存在干扰素-γ的情况下，对植物血凝素（PHA）或白细胞介素-4刺激的单核细胞培养物进行研究。通过酶联免疫吸附测定法测量培养上清液中释放的可溶性CD23（sCD23）。PHA和白细胞介素-4均可诱导特应性和非特应性个体淋巴细胞上CD23的表达。PHA可增加CD23+B细胞和T细胞的百分比及平均荧光强度，而单独的白细胞介素-4不会增加CD23+T细胞的百分比。两组中干扰素-γ的作用不同，因为它仅能降低非特应性个体中PHA刺激的CD23+T细胞的百分比。在非特应性个体中，而非特应性个体中，PHA和白细胞介素-4培养上清液中的sCD23水平升高。这些结果表明，CD23在B细胞和T细胞上的表达调节不同，且干扰素-γ在特应性和非特应性个体中的作用不同。
