[从人乳脂肪球膜中分离乳腺上皮细胞膜抗原]

[Isolation of membrane antigens of mammary gland epithelial cells from membranes of human milk fat globules].

作者信息

Iakubovskaia R I, Korosteleva M D, Aleksander S K, Avdeev G I

出版信息

Vopr Med Khim. 1986 Sep-Oct;32(5):36-41.

Abstract

Seven antigens, belonging to various structures of bilayer: ArI (lactoferrin) and Ar5 (alpha-lactalbumin) - adsorbed proteins; Ar2 and Ar14 - peripheric components; Ar3, Ar4 and Ar13 - integral structures, were found after treatment of fatty globule membranes from human milk using the agents with improved extracting activity (isotonic solution, 1 M MgCl2, 1% Triton X-100, combined effect of 1% Triton X-100 and sonication). The procedure developed enabled to separate these antigens and to isolate them from the main milk secretory proteins lactoferrin and alpha-lactalbumin. Towards highly purified preparations of the membrane antigens 3 and 4 monospecific antisera were raised, which allowed to identify these components among tissue-specific antigens of mammary gland capable to be maintained in malignization of the tissue. Ar4 was also found in tumoral tissues of ovary, lung and stomach.

摘要

使用具有增强提取活性的试剂（等渗溶液、1 M 氯化镁、1% Triton X-100、1% Triton X-100 与超声处理的联合作用）处理人乳脂肪球膜后，发现了七种属于双层不同结构的抗原：ArI（乳铁蛋白）和 Ar5（α-乳白蛋白）——吸附蛋白；Ar2 和 Ar14——外周成分；Ar3、Ar4 和 Ar13——整合结构。所开发的程序能够分离这些抗原，并将它们与主要的乳分泌蛋白乳铁蛋白和α-乳白蛋白分离。针对膜抗原 3 和 4 的高度纯化制剂制备了单特异性抗血清，这使得能够在乳腺组织特异性抗原中识别这些成分，这些抗原在组织恶性化过程中能够得以保留。在卵巢、肺和胃的肿瘤组织中也发现了 Ar4。