[Isolation, amino acid composition and type of action of gastric secretion inhibitors derived from k-casein].

Yield of an inhibitor of gastric secretion and its activity were increased after additional step of the k-casein purification as well as a result of increase in the enzyme/substrate ratio at the step of enzymatic hydrolysis and of substitution of the filtrate dialysis by extraction with ether. Amino acid analysis of the gel chromatographic fractions of inhibitor demonstrated the presence of para-k-casein fragments in its molecule. As shown by kinetics of inhibitory action the preparation included two inhibitors of gastric secretion. The inhibitor I exhibited its effect immediately after administration and increased the latent period of the gastric acidification. The effect of the inhibitor II was manifested with in 1.5-2 hrs after administration. The preparation isolated may be a useful drug in treatment of gastroduodenal impairments caused by hyperacidic states.