CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisbon, Portugal.
Laboratório Associado para Ciência Animal e Veterinária (AL4AnimalS), Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisbon, Portugal.
Genes (Basel). 2023 Aug 25;14(9):1694. doi: 10.3390/genes14091694.
Pancreatic cancer is anticipated to be the second leading cause of cancer-related death by 2030. Aquaporins (AQPs), a family of water channel proteins, have been linked to carcinogenesis. The aim of this study was to determine AQP gene expression in pancreatic cancer tissues and to validate aquaporins as possible diagnosis and/or prognosis genes. The relative gene expression levels of AQP1, AQP3, AQP5, and AQP9 were analyzed using real-time quantitative PCR (RT-qPCR) in 24 paired pancreatic tumors and adjacent healthy tissues according to variables such as age, gender, and tumor invasiveness and aggressiveness. AQPs transcripts were detected in both healthy and tumor tissues. While AQP1 was downregulated in the tumor samples, AQP3 was particularly overexpressed in low-grade invasive tumors. Interestingly, most of the strong positive Pearson correlation coefficients found between AQPs in healthy tissues were lost when analyzing the tumor tissues, suggesting disruption of the coordinated AQP-gene expression in pancreatic cancer.
预计到 2030 年，胰腺癌将成为癌症相关死亡的第二大主要原因。水通道蛋白（AQPs）是一类水通道蛋白，与癌症的发生有关。本研究旨在确定胰腺癌组织中 AQP 基因的表达，并验证水通道蛋白是否可作为潜在的诊断和/或预后基因。根据年龄、性别和肿瘤侵袭性和侵袭性等变量，使用实时定量 PCR（RT-qPCR）分析了 24 对胰腺肿瘤和相邻健康组织中 AQP1、AQP3、AQP5 和 AQP9 的相对基因表达水平。AQPs 转录本在健康组织和肿瘤组织中均有检测到。虽然 AQP1 在肿瘤样本中下调，但 AQP3 在低级别侵袭性肿瘤中表达特别高。有趣的是，当分析肿瘤组织时，在健康组织中发现的大多数 AQPs 之间强正皮尔逊相关系数丢失，这表明在胰腺癌中协调的 AQP 基因表达受到破坏。
