Huang Pei-Cheng, Grunseich John M, Berg-Falloure Katherine M, Tolley Jordan P, Koiwa Hisashi, Bernal Julio S, Kolomiets Michael V
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843-2132, USA.
Department of Entomology, Texas A&M University, College Station, TX 77843-2475, USA.
Genes (Basel). 2023 Aug 30;14(9):1732. doi: 10.3390/genes14091732.
Foliage-feeding fall armyworm (FAW; ) and root-feeding western corn rootworm (WCR; ) are maize ( L.) pests that cause significant yield losses. Jasmonic acid (JA) plays a pivotal defense role against insects. 12-oxo-phytodienoic acid (12-OPDA) is converted into JA by peroxisome-localized OPDA reductases (OPR). However, little is known about the physiological functions of cytoplasmic OPRs. Here, we show that disruption of reduced wound-induced JA production and defense against FAW while accumulating more JA catabolites. Overexpression of in enhanced JA production and defense against beet armyworm (BAW; ). In addition, double mutants were more susceptible than either single mutant, suggesting that and uniquely and additively contributed to defense. In contrast to the defensive roles of and in leaves, single mutants did not display any alteration in root herbivory defense against WCR. Feeding on double mutants resulted in increased WCR mortality associated with greater herbivory-induced production of insecticidal death acids and ketols. Thus, and cooperatively inhibit the synthesis of these metabolites during herbivory by WCR. We conclude that and regulate JA-mediated resistance in leaves against FAW while suppressing insecticidal oxylipin synthesis in roots during WCR infestation.
取食叶片的草地贪夜蛾（FAW；）和取食根部的西方玉米根虫（WCR；）是玉米（L.）害虫，会导致显著的产量损失。茉莉酸（JA）在抵御昆虫方面发挥着关键的防御作用。12-氧代植物二烯酸（12-OPDA）通过过氧化物酶体定位的OPDA还原酶（OPR）转化为JA。然而，关于细胞质OPR的生理功能知之甚少。在这里，我们表明破坏会降低伤口诱导的JA产生以及对草地贪夜蛾的防御能力，同时积累更多的JA分解代谢物。在中过表达会增强JA产生以及对甜菜夜蛾（BAW；）的防御能力。此外，双突变体比任何一个单突变体都更易受影响，这表明和独特且累加地有助于防御。与和在叶片中的防御作用相反，单突变体在对西方玉米根虫的根部食草动物防御方面没有表现出任何改变。取食双突变体导致西方玉米根虫死亡率增加，这与食草动物诱导产生更多的杀虫死亡酸和酮醇有关。因此，和在西方玉米根虫取食期间协同抑制这些代谢物的合成。我们得出结论，和调节叶片中JA介导的对草地贪夜蛾的抗性，同时在西方玉米根虫侵染期间抑制根部中杀虫氧脂的合成。
