Liu Lijun, Zhang Yuhan, Tang Chen, Wu Jine, Fu Jingye, Wang Qiang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, College of Agronomy, Sichuan Agricultural University, 611130, Chengdu, China.
College of Life Science, Sichuan Agricultural University, 625014, Yaan, China.
BMC Genomics. 2024 Apr 23;25(1):397. doi: 10.1186/s12864-024-10297-z.
Jasmonate (JA) is the important phytohormone to regulate plant growth and adaption to stress signals. MYC2, an bHLH transcription factor, is the master regulator of JA signaling. Although MYC2 in maize has been identified, its function remains to be clarified.
To understand the function and regulatory mechanism of MYC2 in maize, the joint analysis of DAP-seq and RNA-seq is conducted to identify the binding sites and target genes of ZmMYC2. A total of 3183 genes are detected both in DAP-seq and RNA-seq data, potentially as the directly regulating genes of ZmMYC2. These genes are involved in various biological processes including plant growth and stress response. Besides the classic cis-elements like the G-box and E-box that are bound by MYC2, some new motifs are also revealed to be recognized by ZmMYC2, such as nGCATGCAnn, AAAAAAAA, CACGTGCGTGCG. The binding sites of many ZmMYC2 regulating genes are identified by IGV-sRNA.
All together, abundant target genes of ZmMYC2 are characterized with their binding sites, providing the basis to construct the regulatory network of ZmMYC2 and better understanding for JA signaling in maize.
茉莉酸（JA）是调节植物生长和适应胁迫信号的重要植物激素。MYC2是一种bHLH转录因子，是JA信号传导的主要调节因子。虽然玉米中的MYC2已被鉴定，但它的功能仍有待阐明。
为了了解MYC2在玉米中的功能和调控机制，进行了DAP-seq和RNA-seq的联合分析，以鉴定ZmMYC2的结合位点和靶基因。在DAP-seq和RNA-seq数据中总共检测到3183个基因，可能是ZmMYC2的直接调控基因。这些基因参与包括植物生长和胁迫反应在内的各种生物学过程。除了MYC2结合的经典顺式元件如G-box和E-box外，还发现一些新的基序也能被ZmMYC2识别，如nGCATGCAnn、AAAAAAAA、CACGTGCGTGCG。通过IGV-sRNA鉴定了许多ZmMYC2调控基因的结合位点。
总之，ZmMYC2的大量靶基因及其结合位点得到了表征，为构建ZmMYC2调控网络和更好地理解玉米中的JA信号传导提供了基础。
