Guangxi Key Laboratory of Plant Functional Substances and Resources Sustainable Utilization, Guangxi Institute of Botany, Guilin 541006, China.
The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan.
Genes (Basel). 2023 Sep 1;14(9):1749. doi: 10.3390/genes14091749.
has medicinal and ornamental value, but the number of wild populations is decreasing dramatically. Thus, conducting research on its genetic diversity and structure is necessary to provide a basis for its conservation. This study aimed to explore the genetic diversity of the wild plant and protect and optimize its wild resources. The genetic diversity of 133 samples from six wild populations of was analyzed using Inter Simple Sequence Repeat molecular markers to provide a scientific basis for the screening of improved germplasm resources. The results showed that the average number of alleles was 1.765, the average number of effective alleles was 1.424, the average Nei's gene diversity index was 0.252, the average Shannon diversity index was 0.381, and the average percentage of polymorphic loci was 76.499%. The variation within the populations was 77.34%, and the variation between the populations was 22.66%. The gene flow was 1.705, which was greater than 1. The results of the cluster analysis showed that the six populations were mainly divided into four clusters and were not classified according to their geographical location. There was no significant correlation between the geographical location and genetic distance between the populations (r = 0.557, > 0.05). The genetic diversity of is high. Among the six wild populations, the genetic diversity of the Mulun population was the highest and this population can be used as a key protection unit. The study on the genetic diversity of can not only reveal the reasons for the decrease in the number of individuals in the population to a certain extent, and put forward the protection strategy, but also provide a scientific basis for the breeding of excellent seed resources.
具有药用和观赏价值，但野生种群数量正在急剧减少。因此，对其遗传多样性和结构进行研究对于保护具有重要意义。本研究旨在探讨野生植物的遗传多样性，保护和优化其野生资源。采用 ISSR 分子标记对来自六个野生种群的 133 个样本的遗传多样性进行分析，为筛选改良的种质资源提供科学依据。结果表明，平均等位基因数为 1.765，平均有效等位基因数为 1.424，Nei's 基因多样性指数平均值为 0.252，Shannon 多样性指数平均值为 0.381，多态位点百分率平均值为 76.499%。种群内变异为 77.34%，种群间变异为 22.66%。基因流为 1.705，大于 1。聚类分析结果表明，六个种群主要分为四个聚类，而不是根据地理位置进行分类。种群间的地理距离与遗传距离之间无显著相关性（r=0.557， >0.05）。 的遗传多样性较高。在六个野生种群中，木论种群的遗传多样性最高，可作为重点保护单位。对 的遗传多样性研究不仅可以在一定程度上揭示种群个体数量减少的原因，并提出保护策略，还可以为优良种子资源的培育提供科学依据。
