3rd Internal Medicine, Diabetes and Rheumatology Department, University of Medicine and Pharmacy "Victor Babeș", 300041 Timișoara, Romania.
Cardiology Department, University of Medicine and Pharmacy "Victor Babeș", 300041 Timișoara, Romania.
Int J Mol Sci. 2023 Sep 10;24(18):13918. doi: 10.3390/ijms241813918.
To assess the relationship between endothelial dysfunction and serum cytokines, anti-SSA and anti-SSB antibodies, beta-2 microglobulin levels, focus score and EULAR Sjögren's Syndrome Disease Activity Index (ESSDAI) in primary Sjögren's syndrome (pSS) patients. The study included 90 patients with pSS and 45 healthy subjects, matched for age and gender, as controls. Serum beta-2 microglobulin, total cholesterol, HDL-cholesterol, triglycerides, TNF-α, and IL-6 were analyzed in both the groups. Patients with pSS were also tested for antinuclear antibodies, anti-SAA (anti-Sjögren's syndrome-related antigen A) antibodies, anti-SSB (anti-Sjögren syndrome related antigen B) antibodies, and focus score (the histopathologic one, based on minor salivary gland biopsy). Endothelial dysfunction was assessed by means of flow-mediated dilation (FMD) in the brachial artery. Data are presented as mean ± standard deviation. Statistical analysis was performed using the -test and the Pearson's correlation. Differences were considered to be statistically significant if the value of < 0.05. Endothelial dysfunction was identified in pSS patients ( < 0.00001). The serum levels of cytokines (TNF-α, respective IL-6) and beta-2 microglobulin were increased in pSS patients compared with controls ( < 0.00001). Endothelial dysfunction (expressed as FMD%) was correlated with focus score, ESSDAI, levels of anti-SSA and anti-SSB antibodies, beta-2 microglobulin, IL-6, and TNF-α, with statistical significance. Endothelial dysfunction is present in pSS patients and is associated with a high focus score and activity as well as increased concentrations of antibodies, pro-inflammatory cytokines, and beta 2-microglobulin.
评估原发性干燥综合征（pSS）患者的血管内皮功能障碍与血清细胞因子、抗 SSA 和抗 SSB 抗体、β2-微球蛋白水平、焦点评分和 EULAR 干燥综合征疾病活动指数（ESSDAI）之间的关系。该研究纳入了 90 例 pSS 患者和 45 例年龄和性别相匹配的健康对照者。检测两组患者的血清β2-微球蛋白、总胆固醇、高密度脂蛋白胆固醇、甘油三酯、TNF-α和 IL-6。还对 pSS 患者进行了抗核抗体、抗 SAA（抗干燥综合征相关抗原 A）抗体、抗 SSB（抗干燥综合征相关抗原 B）抗体和焦点评分（基于小唾液腺活检的组织病理学评分）检测。通过肱动脉血流介导的舒张（FMD）评估血管内皮功能障碍。数据表示为均数±标准差。采用 t 检验和 Pearson 相关分析进行统计学分析。如果 P 值 < 0.05，则认为差异具有统计学意义。pSS 患者存在血管内皮功能障碍（<0.00001）。与对照组相比，pSS 患者的细胞因子（TNF-α和相应的 IL-6）和β2-微球蛋白血清水平升高（<0.00001）。血管内皮功能障碍（以 FMD%表示）与焦点评分、ESSDAI、抗 SSA 和抗 SSB 抗体水平、β2-微球蛋白、IL-6 和 TNF-α相关，具有统计学意义。pSS 患者存在血管内皮功能障碍，与高焦点评分和高疾病活动度相关，同时伴有抗体、促炎细胞因子和β2-微球蛋白浓度升高。
