State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
Int J Mol Sci. 2023 Sep 15;24(18):14134. doi: 10.3390/ijms241814134.
head blight (FHB) is a global cereal disease caused by a complex of species. Both and are the causal agents of FHB in China. is the predominant species in the Middle-Lower Reaches of the Yangtze River (MLRYR) and southwest China. Therefore, detecting in a timely manner is crucial for controlling the disease and preventing mycotoxins from entering the food chain. Here, we combined rapid genomic DNA extraction, recombinase polymerase amplification, Cas12a cleavage, and lateral flow detection techniques to develop a method for the rapid detection of . The reaction conditions were optimized to provide a rapid, sensitive, and cost-effective method for detection. The optimized method demonstrated exceptional specificity in detecting while not detecting any of the 14 other strains and 3 non- species. Additionally, it could detect DNA at concentrations as low as 20 ag/μL, allowing for the diagnosis of infection in maize and wheat kernels even after 3 days of inoculation. The developed assay will provide an efficient and robust detection platform to accelerate plant pathogen detection.
镰刀菌穗腐病（FHB）是一种由多种物种引起的全球性谷类疾病。 和 都是中国 FHB 的病原体。 在长江中下游（MLRYR）和中国西南部占优势。因此，及时检测 对于控制疾病和防止霉菌毒素进入食物链至关重要。在这里，我们将快速基因组 DNA 提取、重组酶聚合酶扩增、Cas12a 切割和侧向流动检测技术相结合，开发了一种快速检测 的方法。优化了反应条件，为 的检测提供了一种快速、敏感和具有成本效益的方法。优化后的方法在检测 时表现出优异的特异性，而不会检测到其他 14 种 菌株和 3 种非 物种。此外，它可以检测到低至 20 ag/μL 的 DNA，即使在接种后 3 天，也可以诊断玉米和小麦籽粒中的 感染。所开发的检测方法将提供一个高效、强大的检测平台，以加速植物病原体的检测。
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