Marciano Carmela L, Hiland Taylor A, Jackson Krista L, Street Sierra, Maris Carson, Ehrsam Andrew, Hum Julia M, Loghmani Mary Terry, Chu Tien-Min G, Kang Kyung S, Lowery Jonathan W
Division of Biomedical Science, College of Osteopathic Medicine, Marian University, Indianapolis, IN 46222, USA.
Bone & Muscle Research Group, Marian University, Indianapolis, IN 46222, USA.
Int J Mol Sci. 2023 Sep 21;24(18):14392. doi: 10.3390/ijms241814392.
Low back pain (LBP) is a common musculoskeletal complaint that can impede physical function and mobility. Current management often involves pain medication, but there is a need for non-pharmacological and non-invasive interventions. Soft tissue manipulation (STM), such as massage, has been shown to be effective in human subjects, but the molecular mechanisms underlying these findings are not well understood. In this paper, we evaluated potential changes in the soft tissue levels of more than thirty pro- or anti-inflammatory cytokines following instrument-assisted STM (IASTM) in rats with chronic, induced LBP using Complete Freund's Adjuvant. Our results indicate that IASTM is associated with reduced soft tissue levels of Regulated on Activation, Normal T cell Expressed and Secreted (RANTES)/Chemokine (C-C motif) ligand 5 (CCL5) and increased soft tissue levels of Interleukin (IL)-4, which are pro-inflammatory and anti-inflammatory factors, respectively, by 120 min post-treatment. IASTM was not associated with tissue-level changes in C-X-C Motif Chemokine Ligand (CXCL)-5/Lipopolysaccharide-Induced CXC Chemokine (LIX)-which is the murine homologue of IL-8, CXCL-7, Granulocyte-Macrophage-Colony Simulating Factor (GM-CSF), Intercellular Adhesion Molecule (ICAM)-1, IL1-Receptor Antagonist (IL-1ra), IL-6, Interferon-Inducible Protein (IP)-10/CXCL-10, L-selectin, Tumor Necrosis Factor (TNF)-α, or Vascular Endothelial Growth Factor (VEGF) at either 30 or 120 min post-treatment. Combined, our findings raise the possibility that IASTM may exert tissue-level effects associated with improved clinical outcomes and potentially beneficial changes in pro-/anti-inflammatory cytokines in circulation and at the tissue level.
腰痛（LBP）是一种常见的肌肉骨骼疾病，会妨碍身体功能和活动能力。目前的治疗方法通常包括使用止痛药物，但需要非药物和非侵入性的干预措施。软组织手法治疗（STM），如按摩，已被证明对人体有效，但其背后的分子机制尚不清楚。在本文中，我们评估了使用完全弗氏佐剂诱导慢性腰痛的大鼠在接受器械辅助软组织手法治疗（IASTM）后，三十多种促炎或抗炎细胞因子在软组织水平上的潜在变化。我们的结果表明，IASTM与治疗后120分钟时调节激活正常T细胞表达和分泌因子（RANTES）/趋化因子（C-C基序）配体5（CCL5）的软组织水平降低以及白细胞介素（IL）-4的软组织水平升高有关，RANTES/CCL5和IL-4分别是促炎和抗炎因子。IASTM与治疗后30分钟或120分钟时C-X-C基序趋化因子配体（CXCL）-5/脂多糖诱导的CXC趋化因子（LIX，即IL-8的小鼠同源物）、CXCL-7、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、细胞间黏附分子（ICAM）-1、IL-1受体拮抗剂（IL-1ra）、IL-6、干扰素诱导蛋白（IP）-10/CXCL-10、L-选择素、肿瘤坏死因子（TNF）-α或血管内皮生长因子（VEGF）的组织水平变化无关。综合来看，我们的研究结果提出了一种可能性，即IASTM可能会产生与改善临床结果相关的组织水平效应，并可能在循环和组织水平上对促炎/抗炎细胞因子产生有益的变化。
