Anloague Aric, Mahoney Aaron, Ogunbekun Oladipupo, Hiland Taylor A, Thompson William R, Larsen Bryan, Loghmani M Terry, Hum Julia M, Lowery Jonathan W
Division of Biomedical Science, Marian University College of Osteopathic Medicine, 3200 Cold Spring Rd, Indianapolis, IN, 46222, USA.
Bone and Mineral Research Group, Marian University, Indianapolis, IN, 46222, USA.
BMC Res Notes. 2020 Aug 27;13(1):400. doi: 10.1186/s13104-020-05249-1.
Soft tissue manual therapies are commonly utilized by osteopathic physicians, chiropractors, physical therapists and massage therapists. These techniques are predicated on subjecting tissues to biophysical mechanical stimulation but the cellular and molecular mechanism(s) mediating these effects are poorly understood. Previous studies established an in vitro model system for examining mechanical stimulation of dermal fibroblasts and established that cyclical strain, intended to mimic overuse injury, induces secretion of numerous pro-inflammatory cytokines. Moreover, mechanical strain intended to mimic soft tissue manual therapy reduces strain-induced secretion of pro-inflammatory cytokines. Here, we sought to partially confirm and extend these reports and provide independent corroboration of prior results.
Using cultures of primary human dermal fibroblasts, we confirm cyclical mechanical strain increases levels of IL-6 and adding long-duration stretch, intended to mimic therapeutic soft tissue stimulation, after cyclical strain results in lower IL-6 levels. We also extend the prior work, reporting that long-duration stretch results in lower levels of IL-8. Although there are important limitations to this experimental model, these findings provide supportive evidence that therapeutic soft tissue stimulation may reduce levels of pro-inflammatory cytokines. Future work is required to address these open questions and advance the mechanistic understanding of therapeutic soft tissue stimulation.
整骨疗法医生、脊椎按摩师、物理治疗师和按摩治疗师普遍使用软组织手法治疗。这些技术基于对组织施加生物物理机械刺激，但介导这些效应的细胞和分子机制却知之甚少。先前的研究建立了一个体外模型系统来研究真皮成纤维细胞的机械刺激，并确定旨在模拟过度使用损伤的周期性应变会诱导多种促炎细胞因子的分泌。此外，旨在模拟软组织手法治疗的机械应变会减少应变诱导的促炎细胞因子的分泌。在此，我们试图部分证实并扩展这些报告，并为先前的结果提供独立的佐证。
使用原代人真皮成纤维细胞培养物，我们证实周期性机械应变会增加白细胞介素-6（IL-6）的水平，并且在周期性应变后施加旨在模拟治疗性软组织刺激的长时间拉伸会使IL-6水平降低。我们还扩展了先前的研究，报告长时间拉伸会导致白细胞介素-8（IL-8）水平降低。尽管该实验模型存在重要局限性，但这些发现提供了支持性证据，表明治疗性软组织刺激可能会降低促炎细胞因子的水平。需要进一步的研究来解决这些悬而未决的问题，并推进对治疗性软组织刺激机制的理解。
