Prado Caio, Pereira Rodrigo, Durrant Lucia, Júnior Rômulo, Piubeli Francine, Bonfá Maricy
Engineering School of Lorena, University of São Paulo, Lorena 12602-810, Brazil.
Faculty of Biological and Environmental Sciences, Universidade Federal da Grande Dourados, Dourados 79825-070, Brazil.
Life (Basel). 2023 Sep 20;13(9):1935. doi: 10.3390/life13091935.
Fipronil is an insecticide widely used in the agricultural and veterinary sectors for its efficacy in pest control. The presence of fipronil in the environment is mainly due to agricultural and domestic practices and is frequently found in different types of environmental matrices in concentrations ranging from µg/L to mg/L and can be hazardous to non-target organisms due to its high toxicity. This study was carried out to obtain and characterize microorganisms from soil which are capable of biodegrading fipronil that could be of great biotechnological interest. For this purpose, bioprospecting was carried out using fipronil (0.6 g/L) as the main source of carbon and nitrogen for growth. Once obtained, the strain was identified by sequencing the 16S ribosomal RNA (rRNA) gene and the capacity to degrade fipronil was monitored by GC-MS. Our study showed a presence in soil samples of the strain identified as , which was able to metabolize fipronil and its metabolites during the mineralization process. was able to biodegrade fipronil (96%) and its metabolites fipronil-sulfone (92%) and fipronil-sulfide (79%) in 14 days. Overall, the results of this study provided a bacterium with great potential that could contribute to the degradation of fipronil in the environment.
氟虫腈是一种杀虫剂，因其在害虫防治方面的功效而广泛应用于农业和兽医领域。环境中氟虫腈的存在主要归因于农业和家庭活动，并且经常在不同类型的环境基质中被发现，其浓度范围从微克/升到毫克/升，由于其高毒性，可能对非目标生物有害。本研究旨在从土壤中获取并鉴定能够生物降解氟虫腈的微生物，这可能具有重大的生物技术意义。为此，以氟虫腈（0.6克/升）作为生长的主要碳源和氮源进行生物勘探。菌株获得后，通过对16S核糖体RNA（rRNA）基因进行测序来鉴定，并通过气相色谱-质谱联用仪（GC-MS）监测其降解氟虫腈的能力。我们的研究表明，在土壤样品中存在被鉴定为 的菌株，该菌株能够在矿化过程中代谢氟虫腈及其代谢产物。 在14天内能够生物降解氟虫腈（96%）及其代谢产物氟虫腈砜（92%）和氟虫腈硫醚（79%）。总体而言，本研究结果提供了一种具有巨大潜力的细菌，它可能有助于环境中氟虫腈的降解。
