Genomic Library Exploration Enhances Oxidative Stress Resistance and Poly-3-hydroxybutyrate Production in Recombinant .

Cell growth is inhibited by abiotic stresses during industrial processes, which is a limitation of microbial cell factories. Microbes with robust phenotypes are critical for its maximizing the yield of the target products in industrial biotechnology. Currently, there are several reports on the enhanced production of industrial metabolite through the introduction of genes into host cells, which confers cellular robustness. is known for its unique genetic function thriving in extreme environments such as radiation, UV, and oxidants. In this study, we established that showed greater resistance to oxidation and UV-C than commonly used . By screening the genomic library of , we isolated a gene () encoding a response regulator, which not only enhanced oxidative stress, but also promoted the growth of the recombinant strain. The transcription analysis indicated that the heterologous expression of upregulated oxidative-stress-related genes such as C and A, and acetyl-CoA-accumulation-associated genes via S regulon. Deipr_0871 was applied to improve the production of the valuable metabolite, poly-3-hydroxybutyrate (PHB), in the synthetic strain, which lead to the remarkably higher PHB than the control strain. Therefore, the stress tolerance gene from should be used in the modification of for the production of PHB and other biomaterials.