Subekin Alexei, Alieva Rugiya, Kukushkin Vladimir, Oleynikov Ilya, Zavyalova Elena
Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.
Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia.
Nanomaterials (Basel). 2023 Sep 10;13(18):2531. doi: 10.3390/nano13182531.
Surface-enhanced Raman scattering (SERS) is a powerful technique for decoding of 2-5-component mixes of analytes. Low concentrations of analytes and complex biological media are usually non-decodable with SERS. Recognition molecules, such as antibodies and aptamers, provide an opportunity for a specific binding of ultra-low contents of analyte dissolved in complex biological media. Different approaches have been proposed to provide changes in SERS intensity of an external label upon binding of ultra-low contents of the analytes. In this paper, we propose a SERS-based sensor for the rapid and sensitive detection of botulinum toxin type A. The silver nanoisland SERS substrate was functionalized using an aptamer conjugated with a Raman label. The binding of the target affects the orientation of the label, providing changes in an analytical signal. This trick allowed detecting botulinum toxin type A in a one-stage manner without additional staining with a monotonous dose dependence and a limit of detection of 2.4 ng/mL. The proposed sensor architecture is consistent with the multiarray detection systems for multiplex analyses.
表面增强拉曼散射（SERS）是一种用于解析2至5种分析物混合物的强大技术。低浓度的分析物和复杂的生物介质通常无法用SERS进行解析。识别分子，如抗体和适体，为溶解在复杂生物介质中的超低含量分析物的特异性结合提供了机会。人们已经提出了不同的方法，以便在超低含量的分析物结合后改变外部标记物的SERS强度。在本文中，我们提出了一种基于SERS的传感器，用于快速、灵敏地检测A型肉毒杆菌毒素。使用与拉曼标记物共轭的适体对银纳米岛SERS基底进行功能化。靶标的结合会影响标记物的取向，从而使分析信号发生变化。这种方法能够以单步方式检测A型肉毒杆菌毒素，具有单调的剂量依赖性，检测限为2.4 ng/mL。所提出的传感器架构与用于多重分析的多阵列检测系统一致。
