Department of Chemistry, Chung-Ang University, Seoul 06974, Korea.
Department of Bionano Technology, Hanyang University, Ansan 426-791, Korea.
Sensors (Basel). 2019 Sep 21;19(19):4081. doi: 10.3390/s19194081.
Rapid and sensitive detection of botulinum neurotoxins (BoNTs) is important for immediate treatment with proper antitoxins. However, it is difficult to detect BoNTs at the acute phase of infection, owing to its rarity and ambiguous symptoms. To resolve this problem, we developed a surface-enhanced Raman scattering (SERS)-based immunoassay technique for the rapid and sensitive detection of BoNTs. Magnetic beads and SERS nanotags as capture substrates and detection probes, respectively, and Nile Blue A (NBA) and malachite green isothiocyanate (MGITC) as Raman reporter molecules were used for the detection of two different types of BoNTs (types A and B), respectively. The corresponding limits of detection (LODs) were determined as 5.7 ng/mL (type A) and 1.3 ng/mL (type B). Total assay time, including that for immunoreaction, washing, and detection, was less than 2 h.
快速、灵敏地检测肉毒神经毒素（BoNTs）对于及时使用适当的抗毒素进行治疗非常重要。然而，由于其罕见性和症状不明确，在感染的急性期很难检测到 BoNTs。为了解决这个问题，我们开发了一种基于表面增强拉曼散射（SERS）的免疫分析技术，用于快速、灵敏地检测 BoNTs。磁性珠和 SERS 纳米标签分别作为捕获底物和检测探针，Nile Blue A（NBA）和孔雀石绿异硫氰酸盐（MGITC）分别作为两种不同类型 BoNTs（A 型和 B 型）的拉曼报告分子。相应的检测限（LOD）分别确定为 5.7 ng/mL（A 型）和 1.3 ng/mL（B 型）。总分析时间，包括免疫反应、洗涤和检测时间，不到 2 小时。
