Yang Qian, Guo Juan, Long Xiaofu, Pan Chunyang, Liu Guoqin, Peng Jiantao
Guizhou Key Laboratory for Tobacco Quality Research, College of Tobacco Science, Guizhou University, Huaxi District, Guiyang 550025, China.
Nanomaterials (Basel). 2023 Sep 14;13(18):2558. doi: 10.3390/nano13182558.
The synthesis of metal nanomaterials is a timely topic due to their widespread use in fields such as crop protection, the environment, medicine, and engineering. Green synthesis of nanoparticles, which uses plant extracts instead of industrial chemical agents to reduce metal ions, has been developed to decrease costs, reduce pollution, and improve environmental and human health safety. In this paper, silver nanoparticles (AgNPs) were synthesized from the flower extract of . The green synthesized AgNPs were characterized by UV-Vis, FTIR, XRD, SEM, and other technologies. The antifungal activity of the prepared AgNPs against was tested using the plate method, the concentration dilution method, and other methods, and the antioxidant activity of the prepared AgNPs was evaluated by DPPH and hydroxyl free scavenging methods. The results showed that AgNPs synthesized from flower extract have a face-centered cubic structure (fcc), and the average grain size of the nanoparticles is 13 nm; they are also mainly spherical in shape. Additionally, the concentration of AgNPs (ranging from 16 to 128 μg/mL) significantly inhibited the mycelial growth of in comparison to the control. The inhibitory rate gradually increased with increasing AgNP concentration, ranging from 70.64% to 79.60% at a concentration of 128 μg/mL. The minimum inhibitory concentration was observed at 32 μg/mL. AgNPs induced overaccumulation of MDA in , resulting in cell membrane damage and nucleic acid leakage. Moreover, the AgNPs have significant antioxidant properties, which increase with increasing concentration. The clearance rate of DPPH was 25.46 ± 0.90% when the concentration of AgNPs was 8 μg/mL, and the clearance rate of the hydroxyl radical was 28.62 ± 0.59% when the concentration of AgNPs was 128 μg/mL. Thus, the flower extract from holds potential as an environmentally friendly and green alternative for the synthesis of AgNPs, which have antifungal and antioxidant potential.
由于金属纳米材料在作物保护、环境、医学和工程等领域的广泛应用，其合成是一个热门话题。已经开发出利用植物提取物而非工业化学试剂来还原金属离子的纳米颗粒绿色合成方法，以降低成本、减少污染并提高环境和人类健康安全性。在本文中，银纳米颗粒（AgNPs）是由[植物名称]的花提取物合成的。通过紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）等技术对绿色合成的AgNPs进行了表征。使用平板法、浓度稀释法等方法测试了制备的AgNPs对[真菌名称]的抗真菌活性，并通过二苯基苦味酰基自由基（DPPH）和羟基自由基清除法评估了制备的AgNPs的抗氧化活性。结果表明，由[植物名称]花提取物合成的AgNPs具有面心立方结构（fcc），纳米颗粒的平均粒径为13nm；它们的形状也主要为球形。此外，与对照相比，AgNPs浓度（16至128μg/mL）显著抑制了[真菌名称]的菌丝生长。抑制率随AgNP浓度的增加而逐渐升高，在128μg/mL浓度下为70.64%至79.60%。最低抑菌浓度为32μg/mL。AgNPs导致[真菌名称]中丙二醛（MDA）过度积累，导致细胞膜损伤和核酸泄漏。此外，AgNPs具有显著的抗氧化性能，且随浓度增加而增强。当AgNPs浓度为8μg/mL时，DPPH清除率为25.46±0.90%，当AgNPs浓度为128μg/mL时，羟基自由基清除率为28.62±0.59%。因此，[植物名称]花提取物有望成为合成具有抗真菌和抗氧化潜力的AgNPs的环保绿色替代物。
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