Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.
College of Life Science, Shandong Normal University, Jinan 250014, People's Republic of China.
Int J Nanomedicine. 2021 Jan 19;16:481-492. doi: 10.2147/IJN.S283677. eCollection 2021.
Dihydromyricetin (DMY), a natural flavonoid, has reportedly antibacterial, antioxidant, anticancer and other properties. In the present study, DMY was used as a reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs), and the optimal conditions for its synthesis were studied. The DMY-AgNPs were investigated for their DPPH scavenging properties and their potential against human pathogenic and food-borne bacteria viz. (. In addition, DMY-AgNPs also showed excellent inhibitory effects on cancer Hela, HepG2 and MDA-MB-231 cell lines.
The dihydromyricetin-mediated AgNPs (DMY-AgNPs) were characterized by ultraviolet-visible spectrophotometer (UV-Vis spectra), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD). Antioxidant activity of DMY-AgNPs was determined by 1.1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. The antibacterial activity was determined by 96-well plate (AGAR) gradient dilution, while anticancer potential was determined by MTT assay.
The results showed that the dispersion of AgNPs had the maximum UV-visible absorption at about 410 nm. The synthesized nanoparticles were almost spherical. FTIR was used to identify functional groups that may lead to the transformation of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with biological molecules in the extraction solution. The biosynthesized DMY-AgNPs exhibited good antioxidant properties, at various concentrations (0.01-0.1mg/mL), the free radical scavenging rate was about 56-92%. Furthermore, DMY-AgNPs possessed good antibacterial properties against ( at room temperature. The minimum inhibitory concentrations (MIC) were 10 g/L, and 10 g/L, respectively. The bioactivity of DMY-mediated AgNPs was studied using MTT assay against Hela, HepG2 and MDA-MB-231 cancer cell lines, and all showed good inhibitory effects.
The present study provides a green approach for the synthesis of DMY-AgNPs which exhibited stronger antioxidant, antibacterial and anticancer properties compared to the dihydromyricetin. DMY-AgNPs can serve as an economical, efficient, and effective antimicrobial material for its applications in food and pharmaceutical fields.
二氢杨梅素（DMY）是一种天然黄酮类化合物，具有抗菌、抗氧化、抗癌等特性。本研究以 DMY 为还原剂和稳定剂合成了银纳米粒子（AgNPs），并对其合成的最佳条件进行了研究。研究了 DMY-AgNPs 的 DPPH 清除能力及其对人病原和食源性病原体的潜在作用，包括金黄色葡萄球菌（ 。此外，DMY-AgNPs 对宫颈癌 Hela、肝癌 HepG2 和乳腺癌 MDA-MB-231 细胞系也表现出优异的抑制作用。
用紫外-可见分光光度计（UV-Vis 光谱）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、动态光散射（DLS）、傅里叶变换红外光谱（FTIR）、X 射线光电子能谱（XPS）和 X 射线粉末衍射（XRD）对二氢杨梅素介导的银纳米粒子（DMY-AgNPs）进行了表征。通过 1.1-二苯基-2-苦基肼基（DPPH）清除实验测定 DMY-AgNPs 的抗氧化活性。通过 96 孔板（AGAR）梯度稀释法测定抗菌活性，通过 MTT 法测定抗癌潜力。
结果表明，AgNPs 的分散体在约 410nm 处具有最大的紫外-可见吸收。合成的纳米粒子几乎呈球形。FTIR 用于鉴定可能导致金属离子转化为纳米粒子的功能团。结果表明，制备的 AgNPs 被提取溶液中的生物分子包裹。生物合成的 DMY-AgNPs 表现出良好的抗氧化性能，在不同浓度（0.01-0.1mg/mL）下，自由基清除率约为 56-92%。此外，DMY-AgNPs 对 （在室温下具有良好的抗菌性能。最小抑菌浓度（MIC）分别为 10g/L 和 10g/L。采用 MTT 法研究了 DMY 介导的 AgNPs 对 Hela、HepG2 和 MDA-MB-231 癌细胞系的生物活性，均表现出良好的抑制作用。
本研究提供了一种绿色的方法来合成 DMY-AgNPs，与二氢杨梅素相比，DMY-AgNPs 具有更强的抗氧化、抗菌和抗癌特性。DMY-AgNPs 可作为一种经济、高效、有效的抗菌材料，应用于食品和制药领域。
