Department of Biotechnology, Kakatiya University, Warangal, 506009, TS, India.
Department of Biotechnology, Singareni Collieries Women's College, Khammam, Telangana State, India.
Appl Biochem Biotechnol. 2023 Jan;195(1):353-368. doi: 10.1007/s12010-022-04156-4. Epub 2022 Sep 9.
The green synthesis of silver nanoparticles (AgNPs) was considered to be efficacious over other approaches due to their eco-friendliness, cost-effectiveness, and high stability. The biosynthesis of AgNPs was achieved by the reduction of silver nitrate using the aqueous leaf extract of Solanum khasianum. The biosynthesized AgNPs were examined by a color change and UV-Vis spectroscopy with an absorption spectrum at 440 nm. The biomolecules existing in S. khasianum leaf extract accountable for bioreduction and capping of AgNPs were analyzed by FTIR analysis and confirmed the presence of alcohols, phenols, alkanes, carboxylic acid, nitro compounds, and amines. The crystalline nature of Sk-AgNPs with face-centered cubic lattice was confirmed by X-ray diffraction (XRD) spectrum. The average crystallite size of Sk-AgNPs was computed as 15.96 nm. The lattice constant, unit cell volume, and spacing values of Sk-AgNPs were parallel to the values indexed in the Joint Committee on Powder Diffraction Standard of silver (JCPDS-04-0783). Scanning electron microscope (SEM) imaging witnessed the spherical structure of synthesized AgNPs. Energy dispersive X-ray (EDX) spectrum acknowledged the AgNPs fabrication with strong signals of silver atoms at 3 keV energy. The biofabricated Sk-AgNPs showed a photoluminescence (PL) emission spectrum of 445 nm with an excitation at 330 nm. Sk-AgNPs showed considerable DPPH radical scavenging activity (87.98%) than BHT (86.14%) and also exhibited significant antidiabetic activity compared to acarbose. Sk-AgNPs revealed antibacterial potentiality against B. sphaericus, E. coli, S. aureus, and P. fluorescens. Moreover, Sk-AgNPs showed dose-dependent cytotoxicity against MCF-7 cell line. This method of green synthesis would support the eco-friendly fabrication of AgNPs from S. khasianum leaf extract with considerable therapeutic activities.
绿色合成的银纳米粒子（AgNPs）被认为比其他方法更有效，因为它们具有环保、经济高效和高稳定性。AgNPs 的生物合成是通过还原硝酸银来实现的，使用的是 Solanum khasianum 的水提叶提取物。生物合成的 AgNPs 通过颜色变化和紫外可见光谱进行检测，其吸收光谱在 440nm 处。通过傅里叶变换红外（FTIR）分析，分析了存在于 S. khasianum 叶提取物中的生物还原和 AgNPs 封端的生物分子，并证实了醇、酚、烷烃、羧酸、硝基化合物和胺的存在。X 射线衍射（XRD）谱证实了 Sk-AgNPs 具有面心立方晶格的晶体性质。Sk-AgNPs 的平均晶粒尺寸计算为 15.96nm。Sk-AgNPs 的晶格常数、晶胞体积和间距值与银的联合委员会粉末衍射标准索引值（JCPDS-04-0783）平行。扫描电子显微镜（SEM）成像见证了合成 AgNPs 的球形结构。能量色散 X 射线（EDX）光谱证实了 AgNPs 的制造，在 3keV 能量下具有强银原子信号。生物制造的 Sk-AgNPs 在 330nm 激发下显示出 445nm 的光致发光（PL）发射光谱。Sk-AgNPs 对 DPPH 自由基的清除活性（87.98%）高于 BHT（86.14%），并且与阿卡波糖相比，也表现出显著的抗糖尿病活性。Sk-AgNPs 对 B. sphaericus、E. coli、S. aureus 和 P. fluorescens 表现出抗菌潜力。此外，Sk-AgNPs 对 MCF-7 细胞系表现出剂量依赖性细胞毒性。这种绿色合成方法支持从 S. khasianum 叶提取物中以环保的方式制造 AgNPs，同时具有相当的治疗活性。
