Kang Sohi, Kim Hyun-Yong, Lee A Yeong, Kim Hyo Seon, Park Jun Hong, Moon Byeong Cheol, Nam Hyeon Hwa, Chae Sung-Wook, Jung Bokyung, Moon Changjong, Shin In Sik, Kim Joong Sun, Seo Yun-Soo
College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju 61186, Republic of Korea.
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111, Geonjae-ro, Naju-si 58245, Jeollanam-do, Republic of Korea.
Pharmaceutics. 2023 Sep 20;15(9):2355. doi: 10.3390/pharmaceutics15092355.
Asthma is a pulmonary disease induced by the inhalation of aeroallergens and subsequent inappropriate immune responses. (L.) Kuntze has been evaluated as an effective antioxidant supplement produced from bioactive compounds, including flavonoids. In this study, we aimed to determine the effects of (L.) Kuntze extract (CE) on ovalbumin-induced allergic asthma. The components of CE were analyzed using high-performance liquid chromatography (HPLC) chromatogram patterns, and asthmatic animal models were induced via ovalbumin treatment. The antioxidant and anti-inflammatory effects of CE were evaluated using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and nitric oxide (NO) assays. Seven compounds were detected in the CE chromatogram. In the ovalbumin-induced mouse model, CE treatment significantly decreased the inflammation index in the lung tissue. CE also significantly decreased eosinophilia and the production of inflammatory cytokines and OVA-specific IgE in animals with asthma. Collectively, our results indicate that CE has anti-inflammatory and antioxidant activities, and that CE treatment suppresses asthmatic progression, including mucin accumulation, inflammation, and OVA-specific IgE production.
哮喘是一种由吸入气传变应原及随后不适当的免疫反应诱发的肺部疾病。昆特泽（L.）Kuntze已被评估为一种由包括黄酮类化合物在内的生物活性化合物制成的有效抗氧化补充剂。在本研究中，我们旨在确定昆特泽（L.）Kuntze提取物（CE）对卵清蛋白诱导的过敏性哮喘的影响。使用高效液相色谱（HPLC）色谱图分析CE的成分，并通过卵清蛋白处理诱导哮喘动物模型。使用2,2-二苯基-1-苦基肼基水合物（DPPH）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和一氧化氮（NO）测定法评估CE的抗氧化和抗炎作用。在CE色谱图中检测到七种化合物。在卵清蛋白诱导的小鼠模型中，CE处理显著降低了肺组织中的炎症指数。CE还显著降低了哮喘动物的嗜酸性粒细胞增多以及炎症细胞因子和OVA特异性IgE的产生。总体而言，我们的结果表明CE具有抗炎和抗氧化活性，并且CE处理可抑制哮喘进展，包括粘蛋白积累、炎症和OVA特异性IgE产生。
