Badmi Raghuram, Gogoi Anupam, Doyle Prestwich Barbara
School of Biological Earth and Environmental Sciences, University College Cork, T23 TK30 Cork, Ireland.
Department of Molecular Plant Biology, Norwegian Institute of Bioeconomy Research (NIBIO), 1433 Ås, Norway.
Plants (Basel). 2023 Sep 12;12(18):3240. doi: 10.3390/plants12183240.
Strawberry is a high-value commercial crop and a model for the economically important Rosaceae family. Strawberry is vulnerable to attack by many pathogens that can affect different parts of the plant, including the shoot, root, flowers, and berries. To restrict pathogen growth, strawberry produce a repertoire of secondary metabolites that have an important role in defense against diseases. Terpenes, allergen-like pathogenesis-related proteins, and flavonoids are three of the most important metabolites involved in strawberry defense. Genes involved in the biosynthesis of secondary metabolites are induced upon pathogen attack in strawberry, suggesting their transcriptional activation leads to a higher accumulation of the final compounds. The production of secondary metabolites is also influenced by the beneficial microbes associated with the plant and its environmental factors. Given the importance of the secondary metabolite pathways in strawberry defense, we provide a comprehensive overview of their literature and their role in the defense responses of strawberry. We focus on terpenoids, allergens, and flavonoids, and discuss their involvement in the strawberry microbiome in the context of defense responses. We discuss how the biosynthetic genes of these metabolites could be potential targets for gene editing through CRISPR-Cas9 techniques for strawberry crop improvement.
草莓是一种高价值的经济作物，也是重要的蔷薇科植物的模式物种。草莓易受多种病原体的侵袭，这些病原体可影响植株的不同部位，包括茎、根、花和果实。为了限制病原体的生长，草莓会产生一系列次生代谢产物，这些产物在抵御疾病方面发挥着重要作用。萜类化合物、类过敏原病程相关蛋白和黄酮类化合物是参与草莓防御的三种最重要的代谢产物。参与次生代谢产物生物合成的基因在草莓受到病原体攻击时被诱导表达，这表明它们的转录激活会导致最终化合物的积累增加。次生代谢产物的产生还受到与植物相关的有益微生物及其环境因素的影响。鉴于次生代谢产物途径在草莓防御中的重要性，我们对其相关文献及其在草莓防御反应中的作用进行了全面综述。我们重点关注萜类化合物、过敏原和黄酮类化合物，并在防御反应的背景下讨论它们与草莓微生物群的关系。我们还讨论了这些代谢产物的生物合成基因如何可能成为通过CRISPR-Cas9技术进行基因编辑以改良草莓作物的潜在靶点。
