Ovesna Jaroslava, Chrpova Jana, Kolarikova Lucia, Svoboda Pavel, Hanzalova Alena, Palicova Jana, Holubec Vojtech
Crop Research Institute, 161 06 Prague, Czech Republic.
Plants (Basel). 2023 Sep 13;12(18):3258. doi: 10.3390/plants12183258.
Crop Wild Relatives (CWRs), as potential sources of new genetic variants, are being extensively studied to identify genotypes that will be able to confer resistance to biotic stresses. In this study, a collection of barley wild relatives was assessed in the field, and their phenotypic variability was evaluated using a Barley Description List, reflecting the identified ecosites. Overall, the CWRs showed significant field resistance to various fungal diseases. To further investigate their resistance, greenhouse tests were performed, revealing that several CWRs exhibited resistance against , , and G.H. Otth. Additionally, to characterize the genetic diversity within the collection, DNA polymorphisms at 21 loci were examined. We successfully employed barley-specific SSR markers, confirming their suitability for identifying and even , i.e., perennial species. The SSR markers efficiently clustered the investigated collection according to species and ecotypes, similarly to the phenotypic assessment. Moreover, SSR markers associated with disease resistance revealed different alleles in comparison to those found in resistant barley cultivars. Overall, our findings highlight that this evaluated collection of CWRs represents a valuable reservoir of genetic variability and resistance genes that can be effectively utilized in breeding programs.
作物野生近缘种（CWRs）作为新基因变异的潜在来源，正受到广泛研究，以鉴定能够赋予生物胁迫抗性的基因型。在本研究中，对一批大麦野生近缘种进行了田间评估，并使用反映已确定生态位点的《大麦描述清单》对其表型变异性进行了评估。总体而言，CWRs对各种真菌病害表现出显著的田间抗性。为进一步研究其抗性，进行了温室试验，结果表明，几个CWRs对、和G.H. Otth表现出抗性。此外，为了表征该群体内的遗传多样性，检测了21个位点的DNA多态性。我们成功地使用了大麦特异性SSR标记，证实了它们适用于鉴定，甚至，即多年生物种。SSR标记根据物种和生态型有效地将研究群体聚类，与表型评估相似。此外，与抗病性相关的SSR标记与抗性大麦品种中发现的等位基因不同。总体而言，我们的研究结果突出表明，这一经过评估的CWRs群体代表了一个宝贵的遗传变异和抗性基因库，可在育种计划中有效利用。
