Kirkby Karen, Roser Sharlene, Plett Krista
NSW Department Primary Industries, Australian Cotton Research Institute, 21888 Kamilaroi Highway, Narrabri, NSW 2390, Australia.
NSW Department Primary Industries, Elizabeth Macarthur Agricultural Institute, Woodbridge Road, Menangle, NSW 2568, Australia.
Plants (Basel). 2023 Sep 15;12(18):3278. doi: 10.3390/plants12183278.
In greenhouse production, grey mould caused by Pers. is one of the most widespread and damaging diseases affecting medicinal cannabis (MC). Fungicide options to control this disease are extremely limited due to the regulations surrounding fungicides and chemical residues as the product end users are medical patients, often with compromised immune systems. Screening for alternative disease control options, such as biological and organic products, can be time-consuming and costly. Here, we optimise and validate a detached leaf assay as a quick and non-destructive method to evaluate interactions between plants and pathogens, allowing the assessment of potential pathogens' infectivity and product efficacy. We tested eight industrial hemp varieties for susceptibility to infection. Using detached leaves from a susceptible variety, we screened a variety of chemical or organic products for efficacy in controlling the lesion development caused by . A consistent reduction in lesion growth was observed using treatments containing Tau-fluvalinate and Myclobutanil, as well as the softer chemical alternatives containing potassium salts. The performance of treatments was pH-dependent, emphasizing the importance of applying them at optimal pH levels to maximise their effectiveness. The detached leaf assay differentiated varietal susceptibility and was an effective method for screening treatment options for diseases caused by Botrytis. The results from the detached leaf assays gave comparable results to responses tested on whole plants.
在温室生产中，由灰葡萄孢引起的灰霉病是影响药用大麻（MC）的最普遍且具破坏性的病害之一。由于围绕杀菌剂和化学残留的法规限制，且产品最终用户为医疗患者，他们的免疫系统往往较弱，因此控制这种病害的杀菌剂选择极为有限。筛选替代病害控制选项，如生物和有机产品，可能既耗时又昂贵。在此，我们优化并验证了一种离体叶片测定法，作为一种快速且无损的方法来评估植物与病原体之间的相互作用，从而能够评估潜在病原体的感染力和产品功效。我们测试了八个工业大麻品种对感染的易感性。使用来自一个易感品种的离体叶片，我们筛选了多种化学或有机产品对由灰葡萄孢引起的病斑发展的控制效果。使用含有氟胺氰菊酯和腈菌唑的处理方法，以及含有钾盐的较温和化学替代物，观察到病斑生长持续减少。处理效果取决于pH值，这强调了在最佳pH水平下施用它们以最大化其有效性的重要性。离体叶片测定法区分了品种的易感性，是筛选由葡萄孢属引起病害的处理选项的有效方法。离体叶片测定法的结果与在整株植物上测试的反应结果相当。
