Balthazar Carole, Novinscak Amy, Cantin Gabrielle, Joly David L, Filion Martin
Department of Biology, Université de Moncton, Moncton, NB E1A 3E9, Canada.
Institute of Health and Life Sciences, Collège La Cité, Ottawa, ON K1K 4R3, Canada.
Phytopathology. 2022 Mar;112(3):549-560. doi: 10.1094/PHYTO-03-21-0128-R. Epub 2022 Mar 2.
Gray mold caused by is one of the most widespread and damaging diseases in cannabis crops worldwide. With challenging restrictions on pesticide use and few effective control measures, biocontrol agents are needed to manage this disease. The aim of this study was to identify bacterial biocontrol agents with wide-spectrum activity against . and other cannabis fungal pathogens. Twelve and strains were first screened with in vitro confrontational assays against 10 culturable cannabis pathogens, namely . , , , , , , , , sp., and sp. Six strains displaying the highest inhibitory activity, namely LBUM279, FZB42, LBUM1082, LBUM979, LBUM223, and Pf-5, were further assessed in planta where all, except LBUM223, significantly controlled gray mold development on cannabis leaves. Notably, LBUM279 and FZB42 reduced disease severity by at least half compared with water-treated plants and prevented lesion development and/or sporulation up to 9 days after pathogen inoculation. Genomes of LBUM279, LBUM1082, and LBUM979 were sequenced de novo and taxonomic affiliations were determined to ensure nonrelatedness with pathogenic strains. Moreover, the genomes were exempt of detrimental genes encoding major toxins and virulence factors that could otherwise pose a biosafety risk when used on crops. Eighteen gene clusters of potential biocontrol interest were also identified. To our knowledge, this is the first reported attempt to control cannabis fungal diseases in planta by direct antagonism with beneficial bacteria.
由[未提及具体病菌名称]引起的灰霉病是全球大麻作物中分布最广、危害最大的病害之一。由于农药使用受到严格限制且有效防治措施较少，因此需要生物防治剂来管理这种病害。本研究的目的是鉴定对[未提及具体病菌名称]以及其他大麻真菌病原体具有广谱活性的细菌生物防治剂。首先，利用体外对峙试验对12株[未提及具体细菌类型]和[未提及具体细菌类型]菌株进行筛选，以对抗10种可培养的大麻病原体，即[列举了10种病菌名称]。对表现出最高抑制活性的6株菌株，即LBUM279、FZB42、LBUM1082、[未提及具体细菌名称]LBUM979、[未提及具体细菌名称]LBUM223和Pf - 5，在植株上进行了进一步评估，结果发现，除LBUM223外，所有菌株均能显著控制大麻叶片上灰霉病的发展。值得注意的是，与水处理植株相比，LBUM279和FZB42使病害严重程度降低了至少一半，并在病原体接种后长达9天内阻止了病斑发展和/或孢子形成。对LBUM279、LBUM1082和LBUM979的基因组进行了从头测序，并确定了分类归属，以确保与致病菌株无关。此外，这些基因组中没有编码主要毒素和毒力因子的有害基因，否则在作物上使用时可能会带来生物安全风险。还鉴定出了18个具有潜在生物防治意义的基因簇。据我们所知，这是首次报道通过与有益细菌直接拮抗来控制植株上大麻真菌病害的尝试。
