Abu-Helalah Munir, Al-Mnayyis Asma'a, Alzoubi Hamed, Al-Abdallah Ruba, Jdaitawi Hussein, Nafi Omar, Abu-Sal Kamel, Altawalbeh Alaa, Khlaifat Alia, Al-Zayadneh Enas, Almaaitah Ihsan, Borghol Ibrahim, Batarseh Fadi, Okkeh Omar, Dalal Abdallah, Alhendi Ahmad, Almaaitah Mohammad, Al-Lahham Adnan, Gazo Mahmoud, Abu Ekteish Faisal, Elnasser Ziad
Department of Family and Community Medicine, Faculty of Medicine, Jordan University, Amman 11942, Jordan.
Department of Clinical Sciences, College of Medicine, Yarmouk University, Irbid 21163, Jordan.
Vaccines (Basel). 2023 Aug 22;11(9):1396. doi: 10.3390/vaccines11091396.
infections are a major cause of mortality and morbidity worldwide. In Jordan, pneumococcal conjugate vaccines (PCVs) are not included in the national vaccination program. Due to the current availability of several PCVs, including PCV-10, PCV-13, and PCV-15, along with PCV-20, currently undergoing pediatric approvals globally, the decision to introduce PCVs and their selection should be based on valid local data on the common serotypes of .
This cross-sectional study aimed to identify the frequency of serotypes of in children aged below 5 years hospitalized with invasive pneumococcal diseases (IPDs), including pneumonia, septicemia, and meningitis, during the study's duration in representative areas of Jordan. Serotyping for culture-positive cases was based on the capsular reaction test, known as the Quellung reaction. qPCR was conducted on the blood samples of patients with lobar pneumonia identified via X-ray or on cerebrospinal fluid for those with a positive latex agglutination test for .
This study was based on the analysis of the serotypes of 1015 cases among children younger than the age of 5: 1006 cases with pneumonia, 6 cases with meningitis, and 3 cases with septicemia. Only 23 culture-positive cases were identified in comparison to 992 lobar pneumonia cases, which were PCR-positive but culture-negative, with a PCR positivity rate of 92%. Serotypes 6B, 6A, 14, and 19F were the most common serotypes identified in this study, with prevalence rates of 16.45%, 13.60%, 12.12%, and 8.18%, respectively. PCV-10, PCV-13, PCV-15, and PCV-20 coverage rates were 45.32%, 61.87%, 64.14%, and 68.47%, respectively.
To the best of our knowledge, this is the largest prospective study from the Middle East and one of the largest studies worldwide showing the serotypes of . It reveals the urgency for the introduction of a PCV vaccination in Jordan, utilizing recently developed vaccines with a broader serotype coverage.
感染是全球范围内死亡和发病的主要原因。在约旦，肺炎球菌结合疫苗（PCV）未被纳入国家疫苗接种计划。由于目前有几种PCV可供使用，包括PCV - 10、PCV - 13和PCV - 15，以及目前正在全球进行儿科审批的PCV - 20，引入PCV及其选择的决定应基于关于常见血清型的有效本地数据。
这项横断面研究旨在确定在约旦代表性地区研究期间因侵袭性肺炎球菌疾病（IPD）住院的5岁以下儿童中肺炎球菌血清型的频率，IPD包括肺炎、败血症和脑膜炎。对培养阳性病例的血清分型基于荚膜反应试验，即荚膜肿胀反应。对通过X射线确诊的大叶性肺炎患者的血液样本或对肺炎球菌乳胶凝集试验呈阳性的患者的脑脊液进行qPCR检测。
本研究基于对1015例5岁以下儿童肺炎球菌病例血清型的分析：1006例肺炎病例、6例脑膜炎病例和3例败血症病例。与992例PCR阳性但培养阴性的大叶性肺炎病例相比，仅鉴定出23例培养阳性病例，PCR阳性率为92%。血清型6B、6A、14和19F是本研究中鉴定出的最常见血清型，患病率分别为16.45%、13.60%、12.12%和8.18%。PCV - 10、PCV - 13、PCV - 15和PCV - 20的覆盖率分别为45.32%、61.87%、64.14%和68.47%。
据我们所知，这是中东地区规模最大的前瞻性研究之一，也是全球最大的显示肺炎球菌血清型的研究之一。它揭示了在约旦引入PCV疫苗接种的紧迫性，应使用最近开发的具有更广泛血清型覆盖率的疫苗。
Zotero 插件