Dhamayanti Esti, Priyowidodo Dwi, Nurcahyo Wisnu, Firdausy Lintang Winantya
Department of Poultry Health and Disease Management, Veterinary Science Program, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
Vet World. 2023 Aug;16(8):1576-1583. doi: 10.14202/vetworld.2023.1576-1583. Epub 2023 Aug 10.
Blood parasite infections in poultry, such as , are a serious threat to the poultry industry due to their potential to cause economic losses. To date, there has been inadequate research on the morphological and molecular detection of the different species that infect poultry in Indonesia. Therefore, this study aimed to analyze the morphological and molecular characteristics of spp. and the several predisposing factors for infection in layer chickens from three districts of Yogyakarta, Indonesia.
One hundred and five blood samples from layer chickens were collected from 13 farms located in three districts of Yogyakarta (Sleman, Bantul, and Kulon Progo) between September and November 2022. Blood samples were subjected to microscopic and polymerase chain reaction (PCR) analyses. Sequencing was performed using basic local alignment search tools to identify the nucleotide structure of cytochrome b. Phylogenetic analysis of was performed using the MEGA-X software.
Microscopic examination revealed that 17/105 positives (16.19%) were positive for blood parasite infection. Trophozoites, erythrocytic meronts, and microgametocytes of were found in blood samples. Based on the morphological examination, the species found in the samples was close to . Polymerase chain reaction examination revealed that 21/60 samples were positive for (35%). The species identified from the sequenced samples were proven to be . The from Thailand was closely related to samples (99.64%-100%) with a genetic distance of 0%-1%. In addition, age, population, and cage type were not significantly associated with infection.
Based on microscopic and PCR examinations, the Plasmodium species found in the three districts of Yogyakarta was . The genetic distance between samples from the three districts of Yogyakarta was closely related (0%-1%) to from Thailand and Japan. There was no correlation between infection and age, cage type, or population.
家禽血液寄生虫感染，如[具体寄生虫名称未给出]，因其可能造成经济损失，对家禽业构成严重威胁。迄今为止，关于印度尼西亚感染家禽的不同[寄生虫名称未给出]物种的形态学和分子检测研究不足。因此，本研究旨在分析印度尼西亚日惹三个地区蛋鸡中[寄生虫名称未给出]物种的形态学和分子特征以及[寄生虫名称未给出]感染的几个诱发因素。
2022年9月至11月期间，从位于日惹三个地区（斯莱曼、班图尔和库隆普罗戈）的13个养殖场采集了105份蛋鸡血样。血样进行了显微镜检查和聚合酶链反应（PCR）分析。使用基本局部比对搜索工具进行测序以鉴定细胞色素b的核苷酸结构。使用MEGA-X软件对[寄生虫名称未给出]进行系统发育分析。
显微镜检查显示，105份样本中有17份（16.19%）血液寄生虫感染呈阳性。在血样中发现了[寄生虫名称未给出]的滋养体、红细胞裂殖体和小配子体。基于形态学检查，样本中发现的物种与[具体参照物种未给出]相近。聚合酶链反应检测显示，60份样本中有21份（35%）[寄生虫名称未给出]呈阳性。从测序样本中鉴定出的[寄生虫名称未给出]物种经证实为[具体物种未给出]。来自泰国的[具体物种未给出]与样本密切相关（99.64%-100%），遗传距离为0%-1%。此外，年龄、鸡群数量和鸡笼类型与[寄生虫名称未给出]感染无显著关联。
基于显微镜和PCR检查，日惹三个地区发现的疟原虫物种为[具体物种未给出]。日惹三个地区样本与来自泰国和日本的[具体物种未给出]的遗传距离密切相关（0%-1%）。[寄生虫名称未给出]感染与年龄、鸡笼类型或鸡群数量之间无相关性。
