Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
F1000Res. 2023 Jul 26;12:403. doi: 10.12688/f1000research.133479.2. eCollection 2023.
CHCHD10 is a mitochondrial protein, implicated in the regulation of mitochondrial morphology and cristae structure, as well as the maintenance of mitochondrial DNA integrity. Recently discovered to be associated with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) in its mutant form, the scientific community would benefit from the availability of validated anti-CHCHD10 antibodies. In this study, we characterized four CHCHD10 commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. As this study highlights high-performing antibodies for CHCHD10, we encourage readers to use it as a guide to select the most appropriate antibody for their specific needs.
CHCHD10 是一种线粒体蛋白，参与调节线粒体形态和嵴结构，以及维持线粒体 DNA 完整性。最近发现其突变形式与肌萎缩侧索硬化症（ALS）和额颞叶痴呆（FTD）有关，科学界将受益于可用的经过验证的抗 CHCHD10 抗体。在这项研究中，我们使用基于比较敲除细胞系和同基因亲本对照的读出值的标准化实验方案，对四种 CHCHD10 商业抗体进行了 Western Blot、免疫沉淀和免疫荧光分析。由于本研究强调了 CHCHD10 的高性能抗体，我们鼓励读者将其用作指南，为他们的特定需求选择最合适的抗体。
