Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
F1000Res. 2024 Apr 8;12:745. doi: 10.12688/f1000research.133645.2. eCollection 2023.
A member of the RNA-binding protein family, T-cell intracellular antigen-1 (TIA1) regulates mRNA translation and splicing as well as cellular stress by promoting stress granule formation. Variants of the gene have implications in neurogenerative disorders including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Reproducible research on TIA1 would be enhanced with the availability of high-quality anti-TIA1 antibodies. In this study, we characterized twelve TIA1 commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.
RNA 结合蛋白家族的一员，T 细胞内抗原-1（TIA1）通过促进应激颗粒的形成来调节 mRNA 的翻译和剪接以及细胞应激。该基因的变体与神经退行性疾病有关，包括肌萎缩侧索硬化症（ALS）和额颞叶痴呆（FTD）。如果有高质量的抗 TIA1 抗体，那么 TIA1 的可重复性研究将会得到增强。在这项研究中，我们使用基于比较敲除细胞系和同基因亲本对照的读出值的标准化实验方案，对十二种 TIA1 商业抗体进行了 Western Blot、免疫沉淀和免疫荧光分析。我们鉴定出了许多性能优异的抗体，并鼓励读者将本报告作为选择最适合其特定需求的抗体的指南。
