Sullivan Mark V, Fletcher Connor, Armitage Rachel, Blackburn Chester, Turner Nicholas W
Department of Chemistry, Dainton Building, University of Sheffield Brook Hill Sheffield S3 7HF UK
Leicester School of Pharmacy, De Montfort University The Gateway Leicester LE1 9BH UK.
Nanoscale Adv. 2023 Aug 28;5(19):5352-5360. doi: 10.1039/d3na00422h. eCollection 2023 Sep 26.
It is becoming increasingly more significant to detect and separate hormones from water sources, with the development of synthetic recognition materials becoming an emerging field. The delicate nature of biological recognition materials such as the antibodies means the generation of robust viable synthetic alternatives has become a necessity. Molecularly imprinted nanoparticles (NanoMIPs) are an exciting class that has shown promise due the generation of high-affinity and specific materials. While nanoMIPs offer high affinity, robustness and reusability, their production can be tricky and laborious. Here we have developed a simple and rapid microwaveable suspension polymerisation technique to produce nanoMIPs for two related classes of drug targets, Selective Androgen Receptor Modulators (SARMs) and steroids. These nanoMIPs were produced using one-pot microwave synthesis with methacrylic acid (MAA) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as a suitable cross-linker, producing particles of an approximate range of 120-140 nm. With the SARMs-based nanoMIPs being able to rebind 94.08 and 94.46% of their target molecules (andarine, and RAD-140, respectively), while the steroidal-based nanoMIPs were able to rebind 96.62 and 96.80% of their target molecules (estradiol and testosterone, respectively). The affinity of nanoMIPs were investigated using Scatchard analysis, with values of 6.60 × 10, 1.51 × 10, 1.04 × 10 and 1.51 × 10 M, for the binding of andarine, RAD-140, estradiol and testosterone, respectively. While the non-imprinted control polymer (NIP) shows a decrease in affinity with values of 3.40 × 10, 1.01 × 10, 1.83 × 10, and 4.00 × 10 M, respectively. The nanoMIPs also demonstrated good selectivity and specificity of binding the targets from a complex matrix of river water, showing these functional materials offer multiple uses for trace compound analysis and/or sample clean-up.
随着合成识别材料的发展成为一个新兴领域，从水源中检测和分离激素变得越来越重要。诸如抗体等生物识别材料的精细性质意味着生成强大可行的合成替代品已成为必要。分子印迹纳米粒子（NanoMIPs）是一类令人兴奋的材料，由于能够生成高亲和力和特异性的材料而展现出前景。虽然纳米分子印迹聚合物具有高亲和力、稳定性和可重复使用性，但其制备可能棘手且费力。在此，我们开发了一种简单快速的可微波悬浮聚合技术，用于制备针对两类相关药物靶点——选择性雄激素受体调节剂（SARMs）和类固醇的纳米分子印迹聚合物。这些纳米分子印迹聚合物是通过一锅法微波合成制备的，使用甲基丙烯酸（MAA）作为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）作为合适的交联剂，制备出粒径约为120 - 140 nm的颗粒。基于SARMs的纳米分子印迹聚合物能够分别重新结合其目标分子（安达林和RAD - 140）的94.08%和94.46%，而基于类固醇的纳米分子印迹聚合物能够分别重新结合其目标分子（雌二醇和睾酮）的96.62%和96.80%。使用Scatchard分析研究了纳米分子印迹聚合物的亲和力，安达林、RAD - 140、雌二醇和睾酮结合的解离常数分别为6.60×10、1.51×10、1.04×10和1.51×10 M。而非印迹对照聚合物（NIP）的亲和力则有所下降，解离常数分别为3.40×10、1.01×10、1.83×10和4.00×10 M。纳米分子印迹聚合物还展示了从河水复杂基质中结合目标物的良好选择性和特异性，表明这些功能材料在痕量化合物分析和/或样品净化方面具有多种用途。
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