Abate Chiara, Neri Giulia, Scala Angela, Mineo Placido Giuseppe, Fazio Enza, Mazzaglia Antonino, Fragoso Alex, Giuffrè Ottavia, Foti Claudia, Piperno Anna
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, Messina 98166, Italy.
Department of Chemical Sciences, University of Catania, Viale A. Doria 6, Catania 95125, Italy.
ACS Appl Nano Mater. 2023 Sep 11;6(18):17187-17195. doi: 10.1021/acsanm.3c03480. eCollection 2023 Sep 22.
The study reports the use of nanoassembly based on cationic cyclodextrin carbon nanotubes (CNT-CDs) and ferrocenylcarnosine (FcCAR) for electrochemical sensing of Hg(II) in aqueous solution. β-cyclodextrins (CDs) were grafted onto CNTs by a click chemistry reaction between heptakis-(6-azido-6-deoxy)-β-cyclodextrin and alkyne-terminated CNTs. The cationic amine groups on the CD units were produced by the subsequent reduction of the residual nitrogen groups. The chemical composition and morphology of CNT-CDs were analyzed by X-ray photoelectron spectroscopy, scanning electron microscopy, and thermogravimetric analysis. A ,-dimethylformamide dispersion of CNT-CDs was cast on the surface of screen-printed carbon electrodes (SPCEs), and the electrochemical response was evaluated by cyclic voltammetry (CV) using [Fe(CN)] as the redox probe. The ability of SPCE/CNT-CD to significantly enhance the electroactive properties of the redox probe was combined with a suitable recognition element (FcCAR) for Hg(II). The electrochemical response of the CNT-CD/FcCAR nanoassembly was evaluated by CV and electrochemical impedance spectroscopy. The analytical performance of the Hg(II) sensor was evaluated by differential pulsed voltammetry and chronoamperometry. The oxidative peak current showed a linear concentration dependence in the range of 1-100 nM, with a sensitivity of 0.12 μA/nM, a limit of detection of 0.50 nM, and a limit of quantification of 1 nM.
该研究报告了基于阳离子环糊精碳纳米管（CNT - CDs）和二茂铁基肌肽（FcCAR）的纳米组装体用于水溶液中Hg(II)的电化学传感。通过七(6 - 叠氮基 - 6 - 脱氧)-β - 环糊精与炔基封端的碳纳米管之间的点击化学反应，将β - 环糊精（CDs）接枝到碳纳米管上。CD单元上的阳离子胺基团是通过随后对残留氮基团的还原产生的。通过X射线光电子能谱、扫描电子显微镜和热重分析对CNT - CDs的化学成分和形态进行了分析。将CNT - CDs的N,N - 二甲基甲酰胺分散体浇铸在丝网印刷碳电极（SPCEs）表面，并以[Fe(CN)]作为氧化还原探针，通过循环伏安法（CV）评估其电化学响应。SPCE/CNT - CD显著增强氧化还原探针电活性的能力与用于Hg(II)的合适识别元件（FcCAR）相结合。通过CV和电化学阻抗谱评估了CNT - CD/FcCAR纳米组装体的电化学响应。通过差分脉冲伏安法和计时电流法评估了Hg(II)传感器的分析性能。氧化峰电流在1 - 100 nM范围内呈线性浓度依赖性，灵敏度为0.12 μA/nM，检测限为0.50 nM，定量限为1 nM。
