利用多价疫苗传递母源免疫及保护奥利亚罗非鱼子代。
Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, .
机构信息
Aquaculture, Pangkep State Polytechnic of Agriculture, Pangkep, South Sulawesi, 90655, Indonesia.
Agricultural Technology Education, Makassar State University, Makassar, South Sulawesi, Indonesia.
出版信息
F1000Res. 2023 Nov 10;10:966. doi: 10.12688/f1000research.52932.3. eCollection 2021.
BACKGROUND
Vaccination is an effective and alternative means of disease prevention, however, it cannot be conducted on the offspring of fish. For this process to take place, the transfer of maternal immunity should be implemented. This study aims to determine the effectiveness of transferring immunity from the broodstock to the offspring using a polyvalent vaccine against and in Nile tilapia,
METHODS
Nile tilapia broodstock with an average weight of 203g (±SD 23) was reared in spawning ponds until mass spawning and harvested one week post-spawning for vaccination. After being vaccinated according to the treatment, each fish broodstock was reared in 3x3 m cages installed in an earthen pond with a density of 20 broodstock, consisting of 15 females and 5 males. The vaccine used was a formalin-killed whole-cell vaccine at a density of 10 cfu/mL injected intramuscularly ( ) at a dose of 0.4 mL/kg fish. Nile tilapia was injected with a vaccine used as a treatment. Example include monovalent (MA) monovalent (MS) monovalent (MP), and bivalent (BAS) and bivalent (BAP), and bivalent (BPS), and and polyvalent vaccines (PAPS). While the control was fish that were injected with a PBS solution. The broodstock's immune response was observed on the 7 , 14 , 21 , and 28 days, while the immune response and challenge test on the offspring was conducted on the 10 , 20 , 30 , and 40 day during the post-hatching period. The parameters observed consisted of total leukocytes, phagocytic activity, antibody titer, lysozyme, and relative survival percentage (RPS).
RESULT
The application of PAPS in broodstock could significantly induce the best immune response and immunity to multiple diseases compared to other treatments. The RPS of the PAPS was also higher than the other types of vaccines. This showed that the transfer of immunity from the broodstock to the Nile tilapia offspring could protect it against bacterial diseases such as , , and .
CONCLUSION
The application of polyvalent vaccine vaccines increased the broodstock's immune response and it was transferred to their offsprings. Polyvalent vaccines derived from maternal immunity can protect offspring from disease up to 30 days of age. They were able to produce tilapia seeds that are immune to diseases caused by , and
背景
疫苗接种是一种有效的疾病预防替代手段，但无法在鱼类的后代身上进行。为了实现这一过程，需要进行母源免疫传递。本研究旨在确定使用多价疫苗对尼罗罗非鱼进行 和 母源免疫传递的效果。
方法
选择平均体重为 203g（±SD 23）的尼罗罗非鱼亲鱼，在产卵池中饲养，直至大规模产卵，然后在产卵后一周收获进行疫苗接种。按照处理进行疫苗接种后，将每条亲鱼饲养在安装在土塘中的 3x3 m 笼中，密度为 20 尾亲鱼，其中包括 15 尾雌鱼和 5 尾雄鱼。使用福尔马林灭活的全细胞疫苗，密度为 10 cfu/mL，肌肉内注射（ ），剂量为 0.4 mL/kg 鱼。尼罗罗非鱼注射疫苗作为处理组。示例包括单价（MA）、单价（MS）、单价（MP）、双价（BAS）和 双价（BAP）、双价（BPS）以及多价疫苗（PAPS）。对照组是注射 PBS 溶液的鱼。亲鱼的免疫反应在第 7、14、21 和 28 天观察，而在孵化后第 10、20、30 和 40 天对后代进行免疫反应和挑战测试。观察的参数包括总白细胞数、吞噬活性、抗体滴度、溶菌酶和相对存活率（RPS）。
结果
与其他处理相比，亲鱼中应用多价疫苗（PAPS）可显著诱导最佳的多疾病免疫反应和免疫力。PAPS 的 RPS 也高于其他类型的疫苗。这表明，从亲鱼向尼罗罗非鱼后代传递免疫可以保护其免受细菌疾病的侵害，如 、 和 。
结论
应用多价疫苗可以提高亲鱼的免疫反应，并将其传递给后代。源自母源免疫的多价疫苗可以保护后代免受 30 天龄以下疾病的侵害。它们能够生产出对 和 引起的疾病具有免疫力的罗非鱼种子。
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