China National GeneBank, BGI, Shenzhen, China.
BGI Research, Shenzhen, China.
mSystems. 2023 Oct 26;8(5):e0054823. doi: 10.1128/msystems.00548-23. Epub 2023 Sep 28.
Biofilm formation is a vital factor for the survival and adaptation of bacteria in diverse environmental niches. Experimental evolution combined with the advancement of whole-population genome sequencing provides us a powerful tool to understand the genomic dynamic of evolutionary adaptation to different environments, such as during biofilm development. Previous studies described the genetic and phenotypic changes of selected clones from experimentally evolved and that were adapted under abiotic and biotic biofilm conditions. However, the full understanding of the dynamic evolutionary landscapes was lacking. Furthermore, the differences and similarities of adaptive mechanisms in and were not identified. To overcome these limitations, we performed longitudinal whole-population genome sequencing to study the underlying genetic dynamics at high resolution. Our study provides the first comprehensive mutational landscape of two bacterial species' biofilms that is adapted to an abiotic and biotic surface.
生物膜的形成对于细菌在不同环境小生境中的生存和适应至关重要。实验进化与全种群基因组测序技术的进步为我们提供了一个强大的工具，可以了解基因组在不同环境（如生物膜发育过程中）的适应性进化的动态。以前的研究描述了在非生物和生物膜条件下，经过实验进化选择的克隆的遗传和表型变化。然而，对动态进化景观的全面理解还存在不足。此外，还没有确定 和 中适应机制的差异和相似之处。为了克服这些限制，我们进行了纵向全种群基因组测序，以高分辨率研究潜在的遗传动态。我们的研究提供了第一个适应于非生物和生物表面的两种细菌生物膜的综合突变景观。
