MOE Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunology, Institute of Immunology and College of Veterinary Medicine, Nanjing Agricultural University , Nanjing, China.
J Virol. 2023 Oct 31;97(10):e0059123. doi: 10.1128/jvi.00591-23. Epub 2023 Sep 28.
Alphaviruses threaten public health continuously, and Getah virus (GETV) is a re-emerging alphavirus that can potentially infect humans. Approved antiviral drugs and vaccines against alphaviruses are few available, but several host antiviral factors have been reported. Here, we used GETV as a model of alphaviruses to screen for additional host factors. Tetrachlorodibenzo-p-dioxin-inducible poly(ADP ribose) polymerase was identified to inhibit GETV replication by inducing ubiquitination of the glycoprotein E2, causing its degradation by recruiting the E3 ubiquitin ligase membrane-associated RING-CH8 (MARCH8). Using GETV as a model virus, focusing on the relationship between viral structural proteins and host factors to screen antiviral host factors provides new insights for antiviral studies on alphaviruses.
甲病毒不断威胁着公共卫生，基孔肯雅病毒（GETV）是一种重新出现的甲病毒，有可能感染人类。目前批准的抗甲病毒药物和疫苗很少，但已经报道了几种宿主抗病毒因子。在这里，我们使用 GETV 作为甲病毒的模型来筛选其他宿主因子。四氯二苯并对二恶英诱导的多聚（ADP 核糖）聚合酶通过诱导糖蛋白 E2 的泛素化，导致其通过募集 E3 泛素连接酶膜相关环指蛋白 8（MARCH8）降解，从而被鉴定为抑制 GETV 复制。使用 GETV 作为模型病毒，重点研究病毒结构蛋白与宿主因子之间的关系，筛选抗病毒宿主因子，为甲病毒的抗病毒研究提供了新的见解。
