Zhang Shanxue, Wei Chunle, Yu Lu, Song Baoan
National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025. P. R. China.
J Agric Food Chem. 2023 Oct 11;71(40):14527-14538. doi: 10.1021/acs.jafc.3c05838. Epub 2023 Sep 28.
Vanisulfane is a plant resistance inducer that exhibits potent activity against potato virus Y (PVY), but its mechanism of action against this virus remains unclear. Our results showed that when we used 400 μg/mL of vanisulfane, it provided an impressive level of control (63.55%) against PVY in L. Meanwhile, vanisulfane increased activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and phenylalanine ammonia lyase (PAL) as well as inducing HO accumulation and Ca influx to mediate PVY resistance. Furthermore, combined transcriptome and proteome analyses revealed that vanisulfane upregulated the POD52, APX, and PR-1 genes and proteins in the salicylic acid (SA) signaling pathway. Experiments demonstrated that vanisulfane triggered the accumulation of SA, upregulated the expression of and genes, and induced resistance against PVY in transgenic plants. Consequently, it can be concluded that vanisulfane mediates the SA-dependent signaling pathway to confer PVY resistance in plants.
香草硫醚是一种植物抗性诱导剂，对马铃薯Y病毒（PVY）具有强大的活性，但其对该病毒的作用机制尚不清楚。我们的结果表明，当使用400μg/mL香草硫醚时，它对番茄中的PVY提供了令人印象深刻的防治水平（63.55%）。同时，香草硫醚增加了过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、过氧化物酶（POD）和苯丙氨酸解氨酶（PAL）的活性，并诱导了H2O2积累和Ca2+内流以介导对PVY的抗性。此外，转录组和蛋白质组联合分析表明，香草硫醚上调了水杨酸（SA）信号通路中的POD52、APX和PR-1基因及蛋白质。实验证明，香草硫醚触发了SA的积累，上调了PR-1和PR-2基因的表达，并在转基因番茄植株中诱导了对PVY的抗性。因此，可以得出结论，香草硫醚介导SA依赖的信号通路，赋予植物对PVY的抗性。
