State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China.
J Agric Food Chem. 2022 Mar 30;70(12):3618-3632. doi: 10.1021/acs.jafc.2c00659. Epub 2022 Mar 17.
Pepper mild mottle virus (PMMoV), an RNA virus, is one of the most devastating pathogens in pepper crops and has a significant influence on global crop yields. PMMoV poses a major threat to the global shortage of pepper plants and other Solanaceae crops due to the lack of an effective antiviral agent. In this study, we have developed a plant immune inducer (vanisulfane), as a "plant vaccine" that boosts plant immunity against PMMoV, and studied its resistance mechanism. The protective activity of vanisulfane against PMMoV was 59.4%. Vanisulfane can enhance the activity of defense enzymes and improve the content of chlorophyll, flavonoids, and total phenols for removing harmful free radicals from plants. Furthermore, vanisulfane was found to enhance defense genes. Label-free quantitative proteomics would tackle disease resistance pathways of vanisulfane. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, differentially abundant proteins (DAPs) are mainly involved in starch and sucrose metabolism, photosynthesis, MAPK signaling pathway, and oxidative phosphorylation pathway. These results are crucial for the discovery of new pesticides, understanding the improvement of plant immunity and the antiviral activity of plant immune inducers.
胡椒轻斑驳病毒（PMMoV）是一种 RNA 病毒，是辣椒作物中最具破坏性的病原体之一，对全球作物产量有重大影响。由于缺乏有效的抗病毒剂，PMMoV 对全球辣椒植物和其他茄科作物的短缺构成了重大威胁。在这项研究中，我们开发了一种植物免疫诱导剂（vanisulfane），作为一种“植物疫苗”，可以增强植物对 PMMoV 的免疫力，并研究了其抗性机制。Vanisulfane 对 PMMoV 的保护活性为 59.4%。Vanisulfane 可以增强防御酶的活性，提高叶绿素、类黄酮和总酚的含量，从而清除植物体内的有害自由基。此外，vanisulfane 被发现可以增强防御基因。无标记定量蛋白质组学将解决 vanisulfane 的抗病途径。根据京都基因与基因组百科全书（KEGG）途径分析，差异丰度蛋白（DAPs）主要参与淀粉和蔗糖代谢、光合作用、MAPK 信号通路和氧化磷酸化通路。这些结果对于发现新的农药、了解植物免疫的提高和植物免疫诱导剂的抗病毒活性至关重要。
