Bioengineering Research and Development Center, BioIRC, Prvoslava Stojanovica 6, 34 000 Kragujevac, Serbia; Institute for Information Technologies, University of Kragujevac, Jovana Cvijica, 34 000 Kragujevac, Serbia; Belgrade Metropolitan University, Tadeuša Košćuška 63, 11158 Belgrade, Serbia.
Bioengineering Research and Development Center, BioIRC, Prvoslava Stojanovica 6, 34 000 Kragujevac, Serbia; Institute for Information Technologies, University of Kragujevac, Jovana Cvijica, 34 000 Kragujevac, Serbia.
Comput Methods Programs Biomed. 2023 Dec;242:107810. doi: 10.1016/j.cmpb.2023.107810. Epub 2023 Sep 12.
We used a 2D fluid-solid interaction (FSI) model to investigate the critical conditions for the arrest of the CTCs traveling through the narrowed capillary with a platelet attached to the capillary wall. This computational model allows us to determine the deformations and the progression of the passage of the CTC through different types of microvessels with platelet included.
The modeling process is obtained using the strong coupling approach following the remeshing procedure. Also, the 1D FE rope element for simulating active ligand-receptor bonds is implemented in our computational tool (described below).
A relationship between the CTCs properties (size and stiffness), the platelet size and stiffness, and the ligand-receptor interaction intensity, on one side, and the time in contact between the CTCs and platelet and conditions for the cell arrest, on the other side, are determined. The model is further validated in vitro by using a microfluidic device with metastatic breast tumor cells.
The computational framework that is presented, with accompanying results, can be used as a powerful tool to study biomechanical conditions for CTCs arrest in interaction with platelets, giving a prognosis of disease progression.
我们使用二维流固耦合（FSI）模型来研究带有附着在血管壁上的血小板的 CTC 通过狭窄毛细血管时停止的临界条件。该计算模型使我们能够确定 CTC 通过包含血小板的不同类型微血管的变形和通过情况。
该建模过程是通过重新划分程序获得的强耦合方法。此外，还在我们的计算工具（如下所述）中实现了用于模拟活性配体-受体键的 1D FE 绳索元素。
确定了 CTC 特性（大小和刚度）、血小板大小和刚度以及配体-受体相互作用强度与 CTC 和血小板之间的接触时间和细胞停止条件之间的关系。该模型进一步通过使用带有转移性乳腺癌细胞的微流控装置进行了体外验证。
提出的计算框架及其伴随的结果可作为研究 CTC 与血小板相互作用时停止的生物力学条件的有力工具，为疾病进展提供预后。
