College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Agricultural Internet of Things, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi 712100, China; Shaanxi Key Laboratory of Agricultural Information Perception and Intelligent Service, Yangling, Shaanxi 712100, China.
Food Chem. 2024 Mar 1;435:137578. doi: 10.1016/j.foodchem.2023.137578. Epub 2023 Sep 24.
Consumption of leafy vegetables with excessive imidacloprid (IMI) can cause serious harm to the human body. To achieve rapid IMI detection, a carbon dots (CDs)-based fluorescent (FL) probe was hydrothermally prepared using O-phenylenediamine as the precursor. The morphology, particle size distribution, crystal structure, optics and chemical bond state of the as-prepared CDs were characterized. The mechanism of the CDs in detecting IMI was investigated by Fourier transform infrared spectroscopy, and the CDs' selectivity, stability, sensitivity, and actual sample recovery were tested. The CDs showed good selectivity, stability, and anti-interference ability. Under optimum conditions, there was a strong linear relationship between the FL intensity of the CDs and the IMI concentration in the range of 0.037-0.2 mg/L. The detection limit was 0.00187 mg/kg. The CDs were successfully applied to detect IMI in lettuce, cole, spinach, and pakchoi with spiked recoveries between 81.026% and 106.803% and a relative standard deviation between 0.001 and 0.027%.
食用含有过量吡虫啉 (IMI) 的叶类蔬菜会对人体造成严重伤害。为了实现快速 IMI 检测，采用邻苯二胺为前驱体制备了基于碳点 (CDs) 的荧光 (FL) 探针。对所制备的 CDs 的形貌、粒径分布、晶体结构、光学和化学键状态进行了表征。通过傅里叶变换红外光谱研究了 CDs 检测 IMI 的机理，并测试了 CDs 的选择性、稳定性、灵敏度和实际样品回收率。CDs 表现出良好的选择性、稳定性和抗干扰能力。在最佳条件下，CDs 的 FL 强度与 IMI 浓度在 0.037-0.2 mg/L 范围内呈强线性关系。检测限为 0.00187 mg/kg。该 CDs 成功应用于生菜、羽衣甘蓝、菠菜和小白菜中 IMI 的检测，加标回收率在 81.026%至 106.803%之间，相对标准偏差在 0.001 至 0.027%之间。
