Department of Marine Biology, Texas A&M University at Galveston, 1001 Texas Clipper Road, Galveston, TX 77554, United States.
Department of Marine Biology, Texas A&M University at Galveston, 1001 Texas Clipper Road, Galveston, TX 77554, United States.
Mol Phylogenet Evol. 2023 Dec;189:107933. doi: 10.1016/j.ympev.2023.107933. Epub 2023 Sep 26.
As some of the smallest vertebrates, yet largest producers of consumed reef biomass, cryptobenthic reef fishes serve a disproportionate role in reef ecosystems and are one of the most poorly understood groups of fish. The blenny genera Hypleurochilus and Parablennius are currently considered paraphyletic and the interrelationships of Parablennius have been the focus of recent phylogenetic studies. However, the interrelationships of Hypleurochilus remain understudied. This genus is transatlantically distributed and comprises 11 species with a convoluted taxonomic history. In this study, relationships for ten Hypleurochilus species are resolved using multi-locus nuclear and mtDNA sequence data, morphological data, and mined COI barcode data. Mitochondrial and nuclear sequence data from 61 individuals collected from the western Atlantic and northern Gulf of Mexico (N. GoM) delimit seven species into a temperate clade, a tropical clade, and a third distinct lineage. This lineage, herein referred to as H. cf. aequipinnis, may represent a species of Hypleurochilus whose range has expanded into the N. GoM. Inclusion of publicly available COI sequence for an additional three species provides further phylogenetic resolution. H. bananensis forms a new eastern Atlantic clade with H. cf. aequipinnis, providing further evidence for a western Atlantic range expansion. Single marker COI delimitation was unable to elucidate the relationships between H. springeri/H. pseudoaequipinnis and between H. multifilis/H. caudovittatus due to incomplete lineage sorting. Mitochondrial data are also unable to accurately resolve the placement of H. bermudensis. However, a comprehensive approach using multi-locus phylogenetic and species delimitation methods was able to resolve these relationships. While mining publicly available sequence data allowed for the inclusion of an increased number of species in the analysis and a more comprehensive phylogeny, it was not without drawbacks, as a handful of sequences are potentially mis-identified. Overall, we find that the recent divergence of some species within this genus and potential introgression events confound the results of single locus delimitation methods, yet a combination of single and multi-locus analyses has allowed for insights into the biogeography of this genus and uncovered a potential transatlantic range expansion.
作为最小的脊椎动物之一，却是消耗的珊瑚礁生物量的最大生产者，cryptobenthic 礁鱼在珊瑚礁生态系统中起着不成比例的作用，是鱼类中最不为人知的群体之一。目前，拟鮨属和副鮨属被认为是并系的，而副鮨属的种间关系一直是最近系统发育研究的焦点。然而，Hypleurochilus 的种间关系仍未得到充分研究。该属分布横跨大西洋，由 11 个种组成，具有复杂的分类历史。在这项研究中，使用多基因核和 mtDNA 序列数据、形态数据和挖掘的 COI 条码数据，对 10 种 Hypleurochilus 种的关系进行了解析。从大西洋西部和墨西哥湾北部（N. GoM）收集的 61 个个体的线粒体和核序列数据将 7 个种限定在一个温带分支、一个热带分支和第三个独特的谱系中。这个谱系，本文称为 H. cf. aequipinnis，可能代表 Hypleurochilus 的一个种，其范围已扩展到 N. GoM。包括另外三个种的公开 COI 序列进一步提高了系统发育分辨率。H. bananensis 与 H. cf. aequipinnis 一起形成了一个新的大西洋东部分支，为大西洋西部范围的扩大提供了进一步的证据。由于不完全谱系分选，单标记 COI 界定无法阐明 H. springeri/H. pseudoaequipinnis 之间以及 H. multifilis/H. caudovittatus 之间的关系。线粒体数据也无法准确解析 H. bermudensis 的位置。然而，使用多基因系统发育和物种界定方法的综合方法能够解决这些关系。虽然挖掘公开可用的序列数据允许在分析中增加更多的物种，并构建更全面的系统发育，但它并非没有缺点，因为有一些序列可能被错误识别。总的来说，我们发现该属内一些种的近期分化和潜在的基因渗入事件使单基因座界定方法的结果复杂化，但单基因座和多基因座分析的结合，使我们能够深入了解该属的生物地理学，并揭示了潜在的跨大西洋范围扩张。
