Effects of released organic components of solid carbon sources on denitrification performance and the related mechanism.

Here, a hybrid scaffold of polyvinyl alcohol/sodium alginate (PVA/SA) was used to prepare solid carbon sources (SCSs) for treating low carbon/nitrogen wastewater. The four SCSs were divided into two groups, biodegradable polymers group (including polyvinyl alcohol-sodium alginate (PS) and PS-PHBV (PP), and blended SCSs (PS-PHBV-wood chips (PPW) and PS-PHBV-wheat straw (PPS)). After the leaching experiments, no changes occurred in elemental composition and functional groups of the SCSs, and the released dissolved organic matter showed a lower degree of humification and higher content of labile molecules in the blended SCSs groups using EEM and FT-ICR-MS. The denitrification performance of the blended SCSs was higher, with nitrate removal efficiency over 84%. High-throughput sequencing confirmed PPW had the highest alpha-diversity, and the microbial community structure significantly varied among SCSs. Results of functional enzymes and genes show the released carbon components directly affect the NADH level and electron transfer efficiency, ultimately influencing denitrification performance.