Department of Kinesiology and Sport Management, Texas A&M University, College Station, Texas, USA.
Department of Kinesiology and Sport Management, Texas Tech University, Lubbock, Texas, USA.
Physiol Rep. 2023 Oct;11(19):e15824. doi: 10.14814/phy2.15824.
The purpose of this study was to assess the influence of genetic background and sex on nitric oxide (NO)-mediated vasomotor function in arteries from different vascular territories. Vasomotor function was assessed in thoracic aorta, abdominal aorta, carotid arteries, and femoral arteries from the following mouse strains: SJL/J, DBA/2J, NZW/LacJ, and C57BL/6J. Contractile responses were assessed using the α1-adrenergic receptor agonist phenylephrine (PE, 10 -10 M). Vasorelaxation responses were assessed by examining relaxation to an endothelium-dependent vasodilator acetylcholine (ACh, 10 -10 M) and an endothelium-independent vasodilator sodium nitroprusside (SNP, 10 -10 M). To evaluate the role of NO, relaxation responses to ACh and SNP were assessed in the absence or presence of a nitric oxide synthase inhibitor (N omega-nitro-l-arginine methyl ester hydrochloride: 10 M). Vasomotor responses to ACh and PE varied across strains and among the arteries tested with some strains exhibiting artery-specific impairment. Results indicated some concentration-response heterogeneity in response to ACh and SNP between vessels from females and males, but no significant differences in responses to PE. Collectively, these findings indicate that vasomotor responses vary by genetic background, sex, and artery type.
本研究旨在评估遗传背景和性别对不同血管区域动脉中一氧化氮（NO）介导的血管舒缩功能的影响。采用α1-肾上腺素能受体激动剂苯肾上腺素（PE，10 -10 M）评估胸主动脉、腹主动脉、颈总动脉和股动脉的血管收缩反应。通过检测内皮依赖性血管扩张剂乙酰胆碱（ACh，10 -10 M）和内皮非依赖性血管扩张剂硝普钠（SNP，10 -10 M）的舒张反应来评估血管舒张反应。为了评估 NO 的作用，在不存在或存在一氧化氮合酶抑制剂（N ω-硝基-L-精氨酸甲酯盐酸盐：10 M）的情况下，评估了 ACh 和 SNP 的舒张反应。ACh 和 PE 的血管舒缩反应在不同品系和所测试的动脉之间存在差异，有些品系表现出动脉特异性损伤。结果表明，女性和男性的血管对 ACh 和 SNP 的反应存在浓度-反应异质性，但对 PE 的反应无显著差异。综上所述，这些发现表明血管舒缩反应因遗传背景、性别和动脉类型而异。
