Xi Menglu, Liang Dong, Yan Yalu, Duan Sufang, Leng Houxi, Yang Haibing, Shi Xiaojin, Na Xiaona, Yang Yucheng, Yang Celi, Szeto Ignatius Man-Yau, Zhao Ai
Vanke School of Public Health, Tsinghua University, Beijing, China.
China National Center for Food Safety Risk Assessment, Beijing, China.
Front Microbiol. 2023 Sep 13;14:1239501. doi: 10.3389/fmicb.2023.1239501. eCollection 2023.
Proteins in breast milk play an important role in the growth and development of infants. This study aims to explore the correlation between functional proteins in breast milk and the infant gut microbiota.
Twenty-three mothers and their infants were enrolled and breast milk samples and infant fecal samples were collected. Breast milk protein content was determined by UPLC-MS/MS, and 16S rRNA sequencing was employed to analyze the gut microbiota of infant.
The results indicated that the secretory immunoglobulin A (sIgA) content in breast milk was positively correlated with the abundance of . The κ-casein content was positively correlated with the abundance of . The osteopontin (OPN) and lactalbumin contents were positively correlated with the abundance of at 42 days. Functional pathway analysis showed that the OPN and κ-casein contents in breast milk were significantly correlated with amino acid, pyruvate, propionic acid, linoleic acid, and alpha-linolenic acid metabolic pathways in early life.
The results of this study suggest that specific proteins in breast milk can influence the abundance of certain gut microbes in infants, playing an important role in early immune and metabolic development.
母乳中的蛋白质在婴儿的生长发育中起着重要作用。本研究旨在探讨母乳中的功能蛋白与婴儿肠道微生物群之间的相关性。
招募了23对母婴，并采集了母乳样本和婴儿粪便样本。采用超高效液相色谱-串联质谱法（UPLC-MS/MS）测定母乳蛋白质含量，并采用16S rRNA测序分析婴儿的肠道微生物群。
结果表明，母乳中分泌型免疫球蛋白A（sIgA）含量与……的丰度呈正相关。κ-酪蛋白含量与……的丰度呈正相关。骨桥蛋白（OPN）和乳白蛋白含量在42天时与……的丰度呈正相关。功能通路分析表明，母乳中的OPN和κ-酪蛋白含量与生命早期的氨基酸、丙酮酸、丙酸、亚油酸和α-亚麻酸代谢通路显著相关。
本研究结果表明，母乳中的特定蛋白质可影响婴儿肠道中某些微生物的丰度，在早期免疫和代谢发育中发挥重要作用。
