Duan Mingzheng, Yang Chengcui, Bao Liuyuan, Han Duo, Wang Huaizheng, Zhang Yongzhi, Liu Honggao, Yang Shunqiang
Yunnan Key Laboratory of Gastrodia Elata and Fungal Symbiotic Biology, College of Agronomy and Life Sciences, Zhaotong University, Zhaotong, China.
Yunnan Engineering Research Center of Green Planting and Processing of Gastrodia Elata, College of Agronomy and Life Sciences, Zhaotong University, Zhaotong, China.
Front Genet. 2023 Sep 12;14:1251695. doi: 10.3389/fgene.2023.1251695. eCollection 2023.
The properties of paddy field (DT) and dry land (HD) soil and food production can be enhanced by the cultivation of (ME) during the fallow period. However, whether ME cultivation affects the soil health and microbial diversity of paddy fields and drylands during the cultivation period remains unclear, and this has greatly limited the wider use of this cultivation model. Here, we analyzed the soil chemical properties and bacterial diversity (via metabarcoding sequencing) of DT and HD soils following ME cultivation. Our findings indicated that ME cultivation could enhance soil health. The content of soil phosphorus and potassium (K) was increased in DT soil under ME cultivation, and the K content was significantly higher in HD soil than in DT soil under ME cultivation. ME cultivation had a weak effect on alpha diversity, and ME cultivation affected the abundance of some genera of soil bacteria. The cultivation of ME might reduce the methane production capacity of DT soil and enhance the nitrogen cycling process of HD soil based on the results of functional annotation analysis. Network analysis and correlation analysis showed that , , and were the key bacterial genera regulating soil chemical properties in DT soil under ME cultivation, and , , , and were the key taxa associated with the accumulation of K in HD soil. The results of our study will aid future efforts to further improve this cultivation model.
在休耕期种植（ME）可提高稻田（DT）和旱地（HD）土壤的性质及粮食产量。然而，ME种植在种植期是否会影响稻田和旱地的土壤健康及微生物多样性仍不清楚，这极大地限制了该种植模式的广泛应用。在此，我们分析了ME种植后DT和HD土壤的化学性质及细菌多样性（通过宏条形码测序）。我们的研究结果表明，ME种植可增强土壤健康。ME种植下DT土壤中磷和钾（K）的含量增加，且ME种植下HD土壤中的K含量显著高于DT土壤。ME种植对α多样性影响较弱，且ME种植影响了土壤细菌某些属的丰度。基于功能注释分析结果，ME种植可能会降低DT土壤的甲烷产生能力，并增强HD土壤的氮循环过程。网络分析和相关性分析表明，在ME种植下， 、 和 是调节DT土壤化学性质的关键细菌属，而 、 、 和 是与HD土壤中K积累相关的关键分类群。我们的研究结果将有助于未来进一步改进这种种植模式。
